1887

Abstract

In this study, to assess the performance of the AdvanSure Mycobacteria GenoBlot assay (AdvanSure assay), we compared its performance with that of the GenoType Mycobacterium CM/AS assay (GenoType assay) for the identification of non-tuberculous mycobacteria (NTM). Twenty-four reference strains and 103 consecutive clinical NTM isolates were analysed. The accuracy rates for the 24 reference strains were 87.5 and 95.8 % for the AdvanSure and GenoType assays, respectively. For the 103 clinical isolates, a 91.3 % (94/103) concordance rate was observed between the two assays. The majority (7/9) of discrepancies were isolates identified as complex (MAC) by only the AdvanSure assay. All of these isolates except one were confirmed as MAC by sequence-based typing. The AdvanSure assay showed comparable performance to the GenoType assay and can be useful as a routine method for NTM identification in the clinical setting, especially where MAC is the main cause of NTM infection.

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2016-12-16
2024-03-29
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References

  1. Adjemian J., Olivier K. N., Seitz A. E., Holland S. M., Prevots D. R. 2012; Prevalence of nontuberculous mycobacterial lung disease in U.S. Medicare beneficiaries. Am J Respir Crit Care Med 185:881–886 [View Article][PubMed]
    [Google Scholar]
  2. Bártfai Z., Somoskövi A., Ködmön C., Szabó N., Puskás E., Kosztolányi L., Faragó E., Mester J., Parsons L. M., Salfinger M. 2001; Molecular characterization of rifampin-resistant isolates of Mycobacterium tuberculosis from Hungary by DNA sequencing and the line probe assay. J Clin Microbiol 39:3736–3739 [View Article][PubMed]
    [Google Scholar]
  3. CLSI 2008; Interpretive Criteria for Identification of Bacteria and Fungi by DNA Target Sequencing; Approved Guideline. CLSI Document MM18-A. Wayne, PA: Clinical and Laboratory Standards Institute;
  4. De Beenhouwer H., Liang Z., De Rijk P., Van Eekeren C., Portaels F. 1995; Detection and identification of mycobacteria by DNA amplification and oligonucleotide-specific capture plate hybridization. J Clin Microbiol 33:2994–2998[PubMed]
    [Google Scholar]
  5. Della-Latta P. 2010; Digestion-decontamination procedures. In Clinical Microbiology Procedures Handbook pp. 7.2.1.1–7.2.1.7 Washington, DC: ASM Press;
    [Google Scholar]
  6. Franco-Alvarez de Luna F., Ruiz P., Gutiérrez J., Casal M. 2006; Evaluation of the GenoType Mycobacteria Direct assay for detection of Mycobacterium tuberculosis complex and four atypical mycobacterial species in clinical samples. J Clin Microbiol 44:3025–3027 [View Article][PubMed]
    [Google Scholar]
  7. García-Agudo L., Jesús I., Rodríguez-Iglesias M., García-Martos P. 2011; Evaluation of INNO-LiPA mycobacteria v2 assay for identification of rapidly growing mycobacteria. Braz J Microbiol 42:1220–1226 [View Article][PubMed]
    [Google Scholar]
  8. Giannini C., Giannelli F., Monti M., Careccia G., Marrocchi M. E., Laffi G., Gentilini P., Zignego A. L. 1999; Prevalence of mixed infection by different hepatitis C virus genotypes in patients with hepatitis C virus-related chronic liver disease. J Lab Clin Med 134:68–73 [View Article][PubMed]
    [Google Scholar]
  9. Gitti Z., Neonakis I., Fanti G., Kontos F., Maraki S., Tselentis Y. 2006; Use of the GenoType Mycobacterium CM and AS assays to analyze 76 nontuberculous mycobacterial isolates from Greece. J Clin Microbiol 44:2244–2246 [View Article][PubMed]
    [Google Scholar]
  10. Griffith D. E., Aksamit T., Brown-Elliott B. A., Catanzaro A., Daley C., Gordin F., Holland S. M., Horsburgh R., Huitt G. et al. 2007; An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 175:367–416 [View Article][PubMed]
    [Google Scholar]
  11. Griffith D. E. 2010; Nontuberculous mycobacterial lung disease. Curr Opin Infect Dis 23:185–190 [View Article][PubMed]
    [Google Scholar]
  12. Hain Lifescience 2011 GenoType Mycobacterium CM Package Insert Nehren, Germany: Hain Lifescience;
    [Google Scholar]
  13. Hofmann-Thiel S., Turaev L., Alnour T., Drath L., Müllerova M., Hoffmann H. 2011; Multi-centre evaluation of the speed-oligo Mycobacteria assay for differentiation of Mycobacterium spp. in clinical isolates. BMC Infect Dis 11:353 [View Article][PubMed]
    [Google Scholar]
  14. Huh H. J., Park K. S., Jang M. A., Kim J. Y., Kwon H. J., Ki C. S., Lee N. Y. 2014; Evaluation of performance of the Real-Q NTM-ID kit for rapid identification of eight nontuberculous mycobacterial species. J Clin Microbiol 52:4053–4055 [View Article][PubMed]
    [Google Scholar]
  15. Jang M. A., Koh W. J., Huh H. J., Kim S. Y., Jeon K., Ki C. S., Lee N. Y. 2014; Distribution of nontuberculous mycobacteria by multigene sequence-based typing and clinical significance of isolated strains. J Clin Microbiol 52:1207–1212 [View Article][PubMed]
    [Google Scholar]
  16. Kim B. J., Lee S. H., Lyu M. A., Kim S. J., Bai G. H., Chae G. T., Kim E. C., Cha C. Y., Kook Y. H. 1999; Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). J Clin Microbiol 37:1714–1720[PubMed]
    [Google Scholar]
  17. Kim K. B., Park S. G., Park J. S., Lee W., Ha J. S., Ryoo N. H., Jeon D. S., Kim J. R. 2015; First case of pulmonary Mycobacterium parascrofulaceum infection in a patient with bronchiectasis in Korea. Ann Lab Med 35:379–381 [View Article][PubMed]
    [Google Scholar]
  18. Kodana M., Tarumoto N., Kawamura T., Saito T., Ohno H., Maesaki S., Ikebuchi K. 2016; Utility of the MALDI-TOF MS method to identify nontuberculous mycobacteria. J Infect Chemother 22:32–35 [View Article][PubMed]
    [Google Scholar]
  19. Lee A. S., Jelfs P., Sintchenko V., Gilbert G. L. 2009; Identification of non-tuberculous mycobacteria: utility of the GenoType Mycobacterium CM/AS assay compared with HPLC and 16S rRNA gene sequencing. J Med Microbiol 58:900–904 [View Article][PubMed]
    [Google Scholar]
  20. Legrand E., Sola C., Verdol B., Rastogi N. 2000; Genetic diversity of Mycobacterium avium recovered from AIDS patients in the Caribbean as studied by a consensus IS1245-RFLP method and pulsed-field gel electrophoresis. Res Microbiol 151:271–283 [View Article][PubMed]
    [Google Scholar]
  21. LG Life Sciences 2013 AdvanSure™ Mycobacteria GenoBlot Assay Package Insert Seoul, Korea: LG Life Sciences;
    [Google Scholar]
  22. Mäkinen J., Marjamäki M., Marttila H., Soini H. 2006; Evaluation of a novel strip test, GenoType Mycobacterium CM/AS, for species identification of mycobacterial cultures. Clin Microbiol Infect 12:481–483 [View Article][PubMed]
    [Google Scholar]
  23. Mareković I., Bošnjak Z., Jakopović M., Boras Z., Janković M., Popović-Grle S. 2016; Evaluation of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in identification of nontuberculous mycobacteria. Chemotherapy 61:167–170 [View Article][PubMed]
    [Google Scholar]
  24. Padilla E., González V., Manterola J. M., Pérez A., Quesada M. D., Gordillo S., Vilaplana C., Pallarés M. A., Molinos S. et al. 2004; Comparative evaluation of the new version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium assays for identification of Mycobacterium species from MB/BacT liquid cultures artificially inoculated with mycobacterial strains. J Clin Microbiol 42:3083–3088 [View Article][PubMed]
    [Google Scholar]
  25. Park J. H., Shim T. S., Lee S. A., Lee H., Lee I. K., Kim K., Kook Y. H., Kim B. J. 2010; Molecular characterization of Mycobacterium intracellulare-related strains based on the sequence analysis of hsp65, internal transcribed spacer and 16S rRNA genes. J Med Microbiol 59:1037–1043 [View Article][PubMed]
    [Google Scholar]
  26. Pfyffer G. E. 2015; Mycobacterium: general characteristics, laboratory detection, and staining procedures. In Manual of Clinical Microbiology pp. 536–569 Washington, DC: ASM Press;
    [Google Scholar]
  27. Prevots D. R., Marras T. K. 2015; Epidemiology of human pulmonary infection with nontuberculous mycobacteria: a review. Clin Chest Med 36:13–34 [View Article][PubMed]
    [Google Scholar]
  28. Ramis I. B., Cnockaert M., Von Groll A., Mathys V., Simon A., Tortoli E., Palomino J. C., Almeida da Silva P. E., Vandamme P. et al. 2015; Evaluation of the Speed-Oligo Mycobacteria assay for the identification of nontuberculous mycobacteria. J Med Microbiol 64:283–287 [View Article][PubMed]
    [Google Scholar]
  29. Richter E., Rüsch-Gerdes S., Hillemann D. 2006; Evaluation of the GenoType Mycobacterium assay for identification of mycobacterial species from cultures. J Clin Microbiol 44:1769–1775 [View Article][PubMed]
    [Google Scholar]
  30. Rodríguez-Sánchez B., Ruiz-Serrano M. J., Ruiz A., Timke M., Kostrzewa M., Bouza E. 2016; Evaluation of MALDI biotyper Mycobacteria Library v3.0 for identification of nontuberculous mycobacteria. J Clin Microbiol 54:1144–1147 [View Article][PubMed]
    [Google Scholar]
  31. Russo C., Tortoli E., Menichella D. 2006; Evaluation of the new GenoType Mycobacterium assay for identification of mycobacterial species. J Clin Microbiol 44:334–339 [View Article][PubMed]
    [Google Scholar]
  32. Sarkola A., Mäkinen J., Marjamäki M., Marttila H. J., Viljanen M. K., Soini H. 2004; Prospective evaluation of the GenoType assay for routine identification of mycobacteria. Eur J Clin Microbiol Infect Dis 23:642–645 [View Article][PubMed]
    [Google Scholar]
  33. Schweickert B., Goldenberg O., Richter E., Göbel U. B., Petrich A., Buchholz P., Moter A. 2008; Occurrence and clinical relevance of Mycobacterium chimaera sp. nov., Germany. Emerg Infect Dis 14:1443–1446 [View Article][PubMed]
    [Google Scholar]
  34. Simons S., van Ingen J., Hsueh P. R., Van Hung N., Dekhuijzen P. N., Boeree M. J., van Soolingen D. 2011; Nontuberculous mycobacteria in respiratory tract infections, eastern Asia. Emerg Infect Dis 17:343–349 [View Article][PubMed]
    [Google Scholar]
  35. Singh A. K., Maurya A. K., Umrao J., Kant S., Kushwaha R. A., Nag V. L., Dhole T. N. 2013; Role of GenoType(®) Mycobacterium common mycobacteria/additional species assay for rapid differentiation between Mycobacterium tuberculosis complex and different species of non-tuberculous mycobacteria. J Lab Physicians 5:83–89 [View Article][PubMed]
    [Google Scholar]
  36. Somoskövi A., Ködmön C., Lantos A., Bártfai Z., Tamási L., Füzy J., Magyar P., Fuzy J. 2000; Comparison of recoveries of Mycobacterium tuberculosis using the automated BACTEC MGIT 960 system, the BACTEC 460 TB system, and Löwenstein-Jensen medium. J Clin Microbiol 38:2395–2397[PubMed]
    [Google Scholar]
  37. Telenti A., Marchesi F., Balz M., Bally F., Böttger E. C., Bodmer T. 1993; Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31:175–178[PubMed]
    [Google Scholar]
  38. Tortoli E. 2003; Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16:319–354 [View Article][PubMed]
    [Google Scholar]
  39. van Ingen J., Bendien S. A., de Lange W. C., Hoefsloot W., Dekhuijzen P. N., Boeree M. J., van Soolingen D. 2009; Clinical relevance of non-tuberculous mycobacteria isolated in the Nijmegen-Arnhem region, The Netherlands. Thorax 64:502–506 [View Article][PubMed]
    [Google Scholar]
  40. van Ingen J. 2013; Diagnosis of nontuberculous mycobacterial infections. Semin Respir Crit Care Med 34:103–109 [View Article][PubMed]
    [Google Scholar]
  41. Weiss C. H., Glassroth J. 2012; Pulmonary disease caused by nontuberculous mycobacteria. Expert Rev Respir Med 6:597–612 [View Article][PubMed]
    [Google Scholar]
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