1887

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Volume 66, Issue 2

Virulence characteristics of clinical isolates vary with the expression levels of s Free

Abstract

We investigated the expression levels of virulence factors (, and ) in five clinical isolates and in a standard ATCC 19606 strain of to determine their effect on the virulence characteristics of the isolates.

The mRNA levels of and proinflammatory cytokines were analyzed by quantitative real-time PCR. For adherence assay, after human lung epithelial cells (A549) were co-cultured with at 37 °C for 2 h, the cell-adherent bacteria was counted. Pearson correlation analysis was used to compare the mRNA levels, the proinflammatory cytokines and the number of adherent bacteria.

The mRNA levels of in the clinical isolates were higher and similar compared with those in ATCC 19606, whereas the mRNA levels of in the clinical isolates were lower and similar compared with those in ATCC 19606. The mRNA levels of in the clinical isolates were significantly higher than those in ATCC 19606. The number of cell-adherent clinical isolates was higher than that of cell-adherent ATCC 19606. Furthermore, the number of cell-adherent clinical isolates was positively and significantly correlated with mRNA level. The mRNA levels of , and in A549 cells co-cultured with the clinical isolates were lower than those in A549 cells co-cultured with ATCC 19606. Moreover, the mRNA levels of , and were negatively and significantly correlated with those of in the isolates.

These results provide insights into the renewed virulence characteristics of clinical isolates that depend on cell adherence capacity and the expression level of mRNAs.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter baumannii , cell-adherence and outer membrane protein
© 2017 The Authors
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2017-02-01
2024-04-16
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Virulence characteristics of Acinetobacter baumannii clinical isolates vary with the expression levels of omps
J. Med. Microbiol. 66, 203 (2017); https://doi.org/10.1099/jmm.0.000394
/content/journal/jmm/10.1099/jmm.0.000394
/content/journal/jmm/10.1099/jmm.0.000394
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