1887

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Volume 66, Issue 1

Frequency of antiseptic resistance genes and reduced susceptibility to biocides in carbapenem-resistant Free

Abstract

The aim of this study is to determine the frequency of quaternary ammonium compound-resistant genes, including , , , , , , and β-lactamase genes, including , , , , , , and in 51 isolates of carbapenem-resistant collected over a period of 2 years and to determine the MIC of chlorhexidine and quaternary ammonium compound-based disinfectant benzalkonium chloride. The and genes were detected by the PCR method. The MICs were determined using the broth microdilution method. The MIC of benzalkonium chloride was in the range of 4 to 64 µg l and chlorhexidine in the range of 4 to 64 µg l. The gene was detected in 96.07 % (49/51) isolates and in 31.37 % (16/51), in 23.52 % (12/51) and in 13.72 % (7/51). The genes and were not found in any of these strains in this study. The frequency of genes was as follows: 84.31 % (43/51) for , 33.33 % (17/51) for , 27.45 % (14/51) for , 19.61 % (10/51) for , 17.65 % (9/51) for , 11.76 % (6/51) for and 7.8 % (4/51) for the . The genes were negative in all the strains. From the study, we concluded that reduced susceptibility to the two disinfectants, as well as and genes, was prevalent among isolates from the hospital environment. This study may offer hospitals important information about the control of nosocomial infections caused by .

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  • Accepted:
  • Published Online:
Keyword(s): Acinetobacter baumannii , bla genes , disinfectant and qac genes
© 2017 The Authors
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2017-01-01
2024-04-24
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References

  1. Blair JM, Webber MA, Baylay AJ, Ogbolu DO, Piddock LJ. Molecular mechanisms of antibiotic resistance. Nat Rev Microbiol 2015; 13:42–51 [View Article][PubMed]
     [Google Scholar]
  2. Li P, Niu W, Li H, Lei H, Liu W et al. Rapid detection of Acinetobacter baumannii and molecular epidemiology of carbapenem-resistant A. baumannii in two comprehensive hospitals of Beijing, China. Front Microbiol 2015; 6: [View Article]
     [Google Scholar]
  3. Messler S, Malecki M, Fuchs PC, Mattner F. Air contamination with A. baumannii during wound dressing change in burn patients but not in surgical patients. Antimicrob Resist Infect Control 2015; 4:P62 [View Article]
     [Google Scholar]
  4. Zhang C, Cui F, Zeng GM, Jiang M, Yang ZZ et al. Quaternary ammonium compounds (QACs): a review on occurrence, fate and toxicity in the environment. Sci Total Environ 2015; 518-519:352–362 [View Article][PubMed]
     [Google Scholar]
  5. Sidhu MS, Heir E, Leegaard T, Wiger K, Holck A. Frequency of disinfectant resistance genes and genetic linkage with beta-lactamase transposon Tn552 among clinical staphylococci. Antimicrob Agents Chemother 2002; 46:2797–2803[PubMed] [CrossRef]
     [Google Scholar]
  6. Correa JE, de Paulis A, Predari S, Sordelli DO, Jeric PE. First report of qacG, qacH and qacJ genes in Staphylococcus haemolyticus human clinical isolates. J Antimicrob Chemother 2008; 62:956–960 [View Article][PubMed]
     [Google Scholar]
  7. Litake GM, Ghole VS, Niphadkar KB, Joshi SG. Phenotypic ESBL detection in Acinetobacter baumannii: a real challenge. Am J Infect Dis 2015; 11:48–53 [View Article]
     [Google Scholar]
  8. Bjorland J, Steinum T, Sunde M, Waage S, Heir E. Novel plasmid-borne gene qacJ mediates resistance to quaternary ammonium compounds in equine Staphylococcus aureus, Staphylococcus simulans, and Staphylococcus intermedius. Antimicrob Agents Chemother 2003; 47:3046–3052[PubMed] [CrossRef]
     [Google Scholar]
  9. Costa SS, Ntokou E, Martins A, Viveiros M, Pournaras S et al. Identification of the plasmid-encoded qacA efflux pump gene in meticillin-resistant Staphylococcus aureus (MRSA) strain HPV107, a representative of the MRSA Iberian clone. Int J Antimicrob Agents 2010; 36:557–561 [View Article][PubMed]
     [Google Scholar]
  10. Zou L, Meng J, Mcdermott PF, Wang F, Yang Q et al. Presence of disinfectant resistance genes in Escherichia coli isolated from retail meats in the USA. J Antimicrob Chemother 2014; 69:2644–2649 [View Article][PubMed]
     [Google Scholar]
  11. Prag G, Falk-Brynhildsen K, Jacobsson S, Hellmark B, Unemo M et al. Decreased susceptibility to chlorhexidine and prevalence of disinfectant resistance genes among clinical isolates of Staphylococcus epidermidis. APMIS 2014; 122:961–967 [View Article]
     [Google Scholar]
  12. Wassenaar TM, Ussery D, Nielsen LN, Ingmer H. Review and phylogenetic analysis of qac genes that reduce susceptibility to quaternary ammonium compounds in Staphylococcus species. Eur J Microbiol Immunol 2015; 5:44–61 [View Article][PubMed]
     [Google Scholar]
  13. Sayeed S, Saunders JR, Edwards C, Corkill JE, Hart CA. Expression of Aeromonas caviae bla genes in Escherichia coli. J Antimicrob Chemother 1996; 38:435–441 [View Article][PubMed]
     [Google Scholar]
  14. Heir E, Sundheim G, Holck AL. Identification and characterization of quaternary ammonium compound resistant staphylococci from the food industry. Int J Food Microbiol 1999; 48:211–219[PubMed] [CrossRef]
     [Google Scholar]
  15. Sidhu MS, Heir E, Sørum H, Holck A. Genetic linkage between resistance to quaternary ammonium compounds and beta-lactam antibiotics in food-related Staphylococcus spp. Microb Drug Resist 2001; 7:363–371 [View Article][PubMed]
     [Google Scholar]
  16. Aygün G, Demirkiran O, Utku T, Mete B, Urkmez S et al. Environmental contamination during a carbapenem-resistant Acinetobacter baumannii outbreak in an intensive care unit. J Hosp Infect 2002; 52:259–262 [View Article][PubMed]
     [Google Scholar]
  17. Garcia LS. Clinical Microbiology Procedures Handbook, 3rd ed. Washington, DC: ASM Press; 2010 [CrossRef]
     [Google Scholar]
  18. Bartual SG, Seifert H, Hippler C, Luzon MA, Wisplinghoff H et al. Development of a multilocus sequence typing scheme for characterization of clinical isolates of Acinetobacter baumannii. J Clin Microbiol 2005; 43:4382–4390 [View Article][PubMed]
     [Google Scholar]
  19. CLSI Performance Standards for Standards for Antimicrobial Susceptibility Testing, Twenty Fifth Informational Supplement. CLSI document M100-S25. Wayne, PA: Clinical and Laboratory Standards Institute; 2015
  20. Babaei M, Sulong A, Hamat R, Nordin S, Neela V. Extremely high prevalence of antiseptic resistant quaternary ammonium compound E gene among clinical isolates of multiple drug resistant Acinetobacter baumannii in Malaysia. Ann Clin Microbiol Antimicrob 2015; 14:11 [View Article][PubMed]
     [Google Scholar]
  21. Long M, Lai H, Deng W, Zhou K, Li B et al. Disinfectant susceptibility of different Salmonella serotypes isolated from chicken and egg production chains. J Appl Microbiol 2016; 121:672–681 [View Article][PubMed]
     [Google Scholar]
  22. Guo L, Long M, Huang Y, Wu G, Deng W et al. Antimicrobial and disinfectant resistance of Escherichia coli isolated from giant pandas. J Appl Microbiol 2015; 119:55–64 [View Article][PubMed]
     [Google Scholar]
  23. Zarrilli R, Casillo R, di Popolo A, Tripodi MF, Bagattini M et al. Molecular epidemiology of a clonal outbreak of multidrug-resistant Acinetobacter baumannii in a university hospital in Italy. Clin Microbiol Infect 2007; 13:481–489 [View Article][PubMed]
     [Google Scholar]
  24. Zmantar T, Kouidhi B, Miladi H, Bakhrouf A. Detection of macrolide and disinfectant resistance genes in clinical Staphylococcus aureus and coagulase-negative staphylococci. BMC Res Notes 2011; 4:453 [View Article][PubMed]
     [Google Scholar]
  25. Furi L, Ciusa ML, Knight D, Di Lorenzo V, Tocci N et al. Evaluation of reduced susceptibility to quaternary ammonium compounds and bisbiguanides in clinical isolates and laboratory-generated mutants of Staphylococcus aureus. Antimicrob Agents Chemother 2013; 57:3488–3497 [View Article][PubMed]
     [Google Scholar]
  26. Mahzounieh M, Khoshnood S, Ebrahimi A, Habibian S, Yaghoubian M. Detection of antiseptic-resistance genes in Pseudomonas and Acinetobacter spp. isolated from burn patients. Jundishapur J Nat Pharm Prod 2014; 9:e15402 [View Article][PubMed]
     [Google Scholar]
  27. Kazama H, Hamashima H, Sasatsu M, Arai T. Distribution of the antiseptic-resistance genes qacE and qacE delta 1 in gram-negative bacteria. FEMS Microbiol Lett 1998; 159:173–178[PubMed]
     [Google Scholar]
  28. Mugnier PD, Poirel L, Naas T, Nordmann P. Worldwide dissemination of the blaOXA-23 carbapenemase gene of Acinetobacter baumannii. Emerg Infect Dis 2010; 16:35–40 [View Article][PubMed]
     [Google Scholar]
  29. Bjorland J, Steinum T, Kvitle B, Waage S, Sunde M et al. Widespread distribution of disinfectant resistance genes among staphylococci of bovine and caprine origin in Norway. J Clin Microbiol 2005; 43:4363–4368 [View Article][PubMed]
     [Google Scholar]
  30. Sidjabat HE, Paterson DL, Adams-Haduch JM, Ewan L, Pasculle AW et al. Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in western Pennsylvania. Antimicrob Agents Chemother 2009; 53:4733–4739 [View Article][PubMed]
     [Google Scholar]
  31. Essack SY, Hall LM, Pillay DG, Mcfadyen ML, Livermore DM. Complexity and diversity of Klebsiella pneumoniae strains with extended-spectrum beta-lactamases isolated in 1994 and 1996 at a teaching hospital in Durban, South Africa. Antimicrob Agents Chemother 2001; 45:88–95 [View Article][PubMed]
     [Google Scholar]
  32. Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 2011; 70:119–123 [View Article][PubMed]
     [Google Scholar]
  33. Qi C, Malczynski M, Parker M, Scheetz MH. Characterization of genetic diversity of carbapenem-resistant Acinetobacter baumannii clinical strains collected from 2004 to 2007. J Clin Microbiol 2008; 46:1106–1109 [View Article][PubMed]
     [Google Scholar]
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Frequency of antiseptic resistance genes and reduced susceptibility to biocides in carbapenem-resistant Acinetobacter baumannii
J. Med. Microbiol. 66, 13 (2017); https://doi.org/10.1099/jmm.0.000403
/content/journal/jmm/10.1099/jmm.0.000403
/content/journal/jmm/10.1099/jmm.0.000403
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