1887

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Volume 66, Issue 1

Recombinant spores expressing cholera toxin B subunit and urease B confer protection against in mice Free

Abstract

infection is associated with chronic gastritis, peptic ulcers, gastric cancer and mucosa-associated lymphoid tissue lymphoma. The limitations of current therapies for infection include poor compliance and antibiotic resistance. Therefore, an effective anti- vaccine would be an alternative or complement to antibiotic treatment. Urease B (UreB) is considered an ideal vaccine antigen against infection. In this study, cholera toxin B subunit (CTB), a mucosal adjuvant, was used to enhance the immunogenicity of a novel spore vaccine expressing CTB-UreB, along with the spore coat protein CotC as a fusion protein. Oral administration of spores expressing CotC-UreB or CotC-CTB-UreB led to increased levels of UreB-specific IgG in serum and UreB-specific IgA in faeces, as well as elevated levels of IL-10 and IFN-γ in splenocytes. In addition, oral administration of CotC-UreB or CotC-CTB-UreB spores induced significant reductions (80.0 and 90.5 %, respectively) in gastric bacterial load (1.11±0.36×10 and 0.53±0.21×10 c.f.u., respectively) compared to that of the CotC control group (5.56±1.64×10 c.f.u., <0.01). Moreover, CotC-CTB-UreB spores were significantly more effective at reducing the bacterial load than CotC-UreB spores (<0.05). These results indicate that CotC-CTB-UreB-expressing spores are a potential vaccine candidate for the control of infection.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus subtilis , cholera toxin B , Helicobacter pylori , recombinant spore , urease B and vaccine
© 2017 The Authors
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2017-01-01
2024-04-19
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Recombinant Bacillus subtilis spores expressing cholera toxin B subunit and Helicobacter pylori urease B confer protection against H. pylori in mice
J. Med. Microbiol. 66, 83 (2017); https://doi.org/10.1099/jmm.0.000404
/content/journal/jmm/10.1099/jmm.0.000404
/content/journal/jmm/10.1099/jmm.0.000404
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