1887

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Volume 66, Issue 3

Pathogenic potential and genotypic diversity of : a neglected food-borne pathogen in Brazil Free

Abstract

is a major zoonotic pathogen that causes food-borne gastroenteritis worldwide. However, there are only a few studies available that have molecularly characterized strains isolated in Brazil. The aim of this study was to genotype 111 strains isolated from sick humans (43), monkey faeces (19), chicken faeces (14), chicken meat (33) and sewage (2) between 1996 and 2016 in Brazil using -SVR (short variable region) sequencing and PFGE. Furthermore, the presence of 16 virulence genes was analysed by PCR.

Using PFGE and -SVR sequencing, the 111 strains studied were grouped into three and two clusters, respectively, and some strains of different origin presented a similarity of ≥80 %. In total, 35 -SVR alleles were detected. Alleles gt45, gt49 and gt57 were the most prevalent, in contrast with those frequently described in the PubMLST database. All 111 strains contained the genes , , , , , , , , , , , , and . The gene was detected in 11 strains (9.9 %), and the in just one strain (0.9 %).

In conclusion, the pathogenic potential of the strains studied was highlighted by the high frequency of the majority of the virulence genes searched. The -SVR sequencing and PFGE results showed that some of the strains studied presented a high genotypic similarity, suggesting potential for transmission between animal sources and humans in this country. Altogether, the results characterize further isolates from Brazil, an important producer and exporter of chicken meat.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Campylobacter jejuni , flaA-SVR sequencing , largest chicken meat exporter , pulsed-field gel electrophoresis and virulence genes
© 2017 The Authors
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2017-03-01
2024-04-18
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Pathogenic potential and genotypic diversity of Campylobacter jejuni: a neglected food-borne pathogen in Brazil
J. Med. Microbiol. 66, 350 (2017); https://doi.org/10.1099/jmm.0.000424
/content/journal/jmm/10.1099/jmm.0.000424
/content/journal/jmm/10.1099/jmm.0.000424
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