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Volume 66, Issue 5

Flagellar expression in clinical isolates of non-typeable Free

Abstract

is a commensal organism found in the upper respiratory tract of humans. When becomes a pathogen, these bacteria can move out of their commensal niche and cause multiple respiratory tract diseases such as otitis media, sinusitis, conjunctivitis and bronchitis in children, and chronic obstructive pulmonary disease in adults. However, is currently considered a non-flagellate bacterium.

In this study, 90 clinical isolates of strains (typeable and non-typeable) showed different degrees of the swarm-motility phenotype

One of these strains, NTHi BUAP96, showed the highest motility rate and its flagella were revealed using transmission electron microscopy and Ryu staining. Moreover, the flagellar genes C and exhibited high homology with those of , and . Furthermore, Western blot analysis, using anti-flagellin heterologous antibodies from , demonstrated cross-reaction with a protein present in NTHi BUAP96.

This study provides, for the first time, information on flagellar expression in , representing an important finding related to its evolution and pathogenic potential.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): flagella , flagellin-like , motility and non-typeable Haemophilus influenzae
© 2017 The Authors
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2017-05-01
2024-04-16
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Flagellar expression in clinical isolates of non-typeable Haemophilus influenzae
J. Med. Microbiol. 66, 592 (2017); https://doi.org/10.1099/jmm.0.000471
/content/journal/jmm/10.1099/jmm.0.000471
/content/journal/jmm/10.1099/jmm.0.000471
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