1887

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Volume 66, Issue 5

DIVA vaccine reduces disease, colonization and shedding due to virulent Typhimurium infection in swine Open Access

Abstract

Non-host-adapted serovars, including the common human food-borne pathogen serovar Typhimurium ( Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA).

An attenuated . Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNA-Seq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains.

Vaccination significantly reduced fever and interferon-gamma (IFNγ) levels in swine challenged with virulent . Typhimurium compared to mock-vaccinated pigs. faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcine-derived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination.

Evaluation of the . Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): animal health , DIVA , food safety , Salmonella , swine and vaccine
© Microbiology Society
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2017-05-01
2024-04-19
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Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine
J. Med. Microbiol. 66, 651 (2017); https://doi.org/10.1099/jmm.0.000482
/content/journal/jmm/10.1099/jmm.0.000482
/content/journal/jmm/10.1099/jmm.0.000482
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