%0 Journal Article %A Hashem, Asmaa A. %A Abd El Fadeal, Noha M. %A Shehata, Atef S. %T In vitro activities of vancomycin and linezolid against biofilm-producing methicillin-resistant staphylococci species isolated from catheter-related bloodstream infections from an Egyptian tertiary hospital %D 2017 %J Journal of Medical Microbiology, %V 66 %N 6 %P 744-752 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000490 %K staphylococci %K linezolid %K vancomycin %K susceptibility %K catheter-related bloodstream infections %K mecA %I Microbiology Society, %X Purpose. Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs. Methodology. Fifty-eight isolates [20 S . aureus and 38 coagulase-negative staphylococci (CoNS; 20 Staphylococcus epidermidis, nine Staphylococcus haemolyticus, three Staphylococcus schleiferi, two Staphylococcus warneri and four Staphylococcus lugdunensis)] were tested for methicillin resistance by cefoxitin disk diffusion and detection of the mecA gene by PCR; biofilm-forming ability using Congo red agar and tissue culture plate methods; susceptibility to ciprofloxacin, clindamycin, cotrimoxazole, erythromycin, gentamicin, linezolid, rifampicin and tetracycline; and MIC determination for vancomycin. Results/Key findings. Cefoxitin resistance was detected among 40 % (8/20) S . aureus isolates, 70 % (14/20) S . epidermidis isolates and 16.7 % (3/18) of other CoNS, although the mecA gene was detected in 45 % (9/20) S . aureus isolates, 35 % (7/20) S . epidermidis isolates and 16.7 % (3/18) of other CoNS. Biofilm-forming ability ranged from 45 to 75 %. Methicillin-resistant S. aureus and other CoNS were considered to be more virulent than methicillin-resistant S. epidermidis due to the higher biofilm forming abilities of the former. All tested isolates exhibited 100 % sensitivity to vancomycin and linezolid, irrespective of their methicillin resistance or biofilm-forming ability. Rifampicin showed overall sensitivity of 75.9 %. Varying degrees of multi-resistance were found for the other antibiotics. Conclusion. Vancomycin, linezolid and rifampicin could be used effectively against methicillin-resistant staphylococci isolated from CRBSIs. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000490