1887

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Volume 66, Issue 10

Discrepant susceptibility to gentamicin despite amikacin resistance in by VITEK 2 represents false susceptibility associated with the 16S rRNA methylase gene Free

Abstract

Because we experienced gentamicin failure in bacteraemia that was susceptible to gentamicin despite amikacin resistance, as determined by VITEK 2, we evaluated the true susceptibility and mechanism of resistance. We screened 2818 isolates during a 1-year period at a university hospital and reviewed anti-microbial susceptibility reports using the VITEK 2 system. The minimum inhibitory concentration was substantiated by broth microdilution (BMD), and the presence of 16S rRNA methylase genes and aminoglycoside-modifying enzymes was also investigated. A total of 131 amikacin-resistant isolates from 19 patients were gentamicin non-resistant according to the VITEK 2 system. Among these, we were able to collect isolates from 12 patients (63.2 %), and a single isolate from each patient was tested. Eleven of the gentamicin non-resistant isolates (91.7 %) showed high-level resistance to both amikacin and gentamicin by BMD in association with the gene. Gentamicin is not an adequate treatment option for amikacin-resistant , even if VITEK 2 reports susceptibility.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): armA , gentamicin , Klebsiella pneumoniae , susceptibility and VITEK 2
© 2017 The Authors
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2017-10-01
2024-04-20
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Discrepant susceptibility to gentamicin despite amikacin resistance in Klebsiella pneumoniae by VITEK 2 represents false susceptibility associated with the armA 16S rRNA methylase gene
J. Med. Microbiol. 66, 1448 (2017); https://doi.org/10.1099/jmm.0.000583
/content/journal/jmm/10.1099/jmm.0.000583
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