1887

Abstract

Lactic acid bacteria (LAB) have been associated with many beneficial effects in human digestive physiology. The aim of this study was to evaluate such effect, including attachment, antiproliferation and anti-pathogenic/antibacterial/antimicrobial properties of LAB isolated from healthy humans.

Thirteen isolates, obtained from fecal samples of healthy individuals, were identified by phenotypic and molecular methods. Human colon adenocarcinoma cell line HT‐29 and the cell proliferation kit II (XTT) assay were used for examination of the adherence and antiproliferative activity, respectively. In addition, the inhibitory effect of isolates against pathogenic bacteria was examined.

Out of 13 isolates, 5 (38 %) isolates were non-adhesive, 4 (31 %) were adhesive and 4 (31 %) were strongly adhesive. Amongst the isolated lactobacilli, showed the highest degree of inhibitory effect against the attachment of the enteropathogens. The XTT assay showed that 3 different isolates had the strongest antiproliferative activity with the maximum effect observed by isolates.

Our results described that different species isolated from normal fecal samples had different degrees of antiproliferative and anti-pathogenic/antibacterial/antimicrobial activities. However, no isolates showed all of the examined properties concurrently, suggestive that a combination of species is needed for an active biological defense system.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.000591
2017-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/66/10/1416.html?itemId=/content/journal/jmm/10.1099/jmm.0.000591&mimeType=html&fmt=ahah

References

  1. Zhang YC, Zhang LW, Tuo YF, Guo CF, Yi HX et al. Inhibition of Shigella sonnei adherence to HT-29 cells by lactobacilli from Chinese fermented food and preliminary characterization of S-layer protein involvement. Res Microbiol 2010; 161:667–672 [View Article][PubMed]
    [Google Scholar]
  2. Živković M, Miljković MS, Ruas-Madiedo P, Markelić MB, Veljović K et al. EPS-SJ exopolisaccharide produced by the strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 is involved in adhesion to epithelial intestinal cells and decrease on E. coli association to Caco-2 cells. Front Microbiol 2016; 7:286 [View Article][PubMed]
    [Google Scholar]
  3. Satish Kumar R, Kanmani P, Yuvaraj N, Paari KA, Pattukumar V et al. Lactobacillus plantarum AS1 binds to cultured human intestinal cell line HT-29 and inhibits cell attachment by enterovirulent bacterium Vibrio parahaemolyticus . Lett Appl Microbiol 2011; 53:481–487 [View Article][PubMed]
    [Google Scholar]
  4. Shokryazdan P, Sieo CC, Kalavathy R, Liang JB, Alitheen NB et al. Probiotic potential of Lactobacillus strains with antimicrobial activity against some human pathogenic strains. Biomed Res Int 2014; 2014:1–16 [View Article][PubMed]
    [Google Scholar]
  5. Gueimonde M, Margolles A, de Los Reyes-Gavilán CG, Salminen S. Competitive exclusion of enteropathogens from human intestinal mucus by Bifidobacterium strains with acquired resistance to bile–a preliminary study. Int J Food Microbiol 2007; 113:228–232 [View Article][PubMed]
    [Google Scholar]
  6. Shanahan F. The colonic microbiota in health and disease. Curr Opin Gastroenterol 2013; 29:49–54 [View Article][PubMed]
    [Google Scholar]
  7. Dhanani AS, Bagchi T. Lactobacillus plantarum CS24.2 prevents Escherichia coli adhesion to HT-29 cells and also down-regulates enteropathogen-induced tumor necrosis factor-α and interleukin-8 expression. Microbiol Immunol 2013; 57:309–315 [View Article][PubMed]
    [Google Scholar]
  8. Rohani M, Noohi N, Talebi M, Katouli M, Pourshafie MR. Highly heterogeneous probiotic Lactobacillus species in healthy iranians with low functional activities. PLoS One 2015; 10:e0144467 [View Article][PubMed]
    [Google Scholar]
  9. Byun R, Nadkarni MA, Chhour KL, Martin FE, Jacques NA et al. Quantitative analysis of diverse Lactobacillus species present in advanced dental caries. J Clin Microbiol 2004; 42:3128–3136 [View Article][PubMed]
    [Google Scholar]
  10. Kwon HS, Yang EH, Yeon SW, Kang BH, Kim TY. Rapid identification of probiotic Lactobacillus species by multiplex PCR using species-specific primers based on the region extending from 16S rRNA through 23S rRNA. FEMS Microbiol Lett 2004; 239:267–275 [View Article][PubMed]
    [Google Scholar]
  11. Chen ZY, Hsieh YM, Huang CC, Tsai CC. Inhibitory effects of probiotic Lactobacillus on the growth of human colonic carcinoma cell line HT-29. Molecules 2017; 22:107 [View Article][PubMed]
    [Google Scholar]
  12. Duary RK, Rajput YS, Batish VK, Grover S. Assessing the adhesion of putative indigenous probiotic lactobacilli to human colonic epithelial cells. Indian J Med Res 2011; 134:664 [View Article][PubMed]
    [Google Scholar]
  13. Candela M, Perna F, Carnevali P, Vitali B, Ciati R et al. Interaction of probiotic Lactobacillus and Bifidobacterium strains with human intestinal epithelial cells: adhesion properties, competition against enteropathogens and modulation of IL-8 production. Int J Food Microbiol 2008; 125:286–292 [View Article][PubMed]
    [Google Scholar]
  14. Grimoud J, Durand H, de Souza S, Monsan P, Ouarné F et al. In vitro screening of probiotics and synbiotics according to anti-inflammatory and anti-proliferative effects. Int J Food Microbiol 2010; 144:42–50 [View Article][PubMed]
    [Google Scholar]
  15. Lee DK, Jang S, Kim MJ, Kim JH, Chung MJ et al. Anti-proliferative effects of Bifidobacterium adolescentis SPM0212 extract on human colon cancer cell lines. BMC Cancer 2008; 8:310 [View Article][PubMed]
    [Google Scholar]
  16. Choi SS, Kim Y, Han KS, You S, Oh S et al. Effects of Lactobacillus strains on cancer cell proliferation and oxidative stress in vitro . Lett Appl Microbiol 2006; 42:452–458 [View Article][PubMed]
    [Google Scholar]
  17. Lee YK, Puong KY, Ouwehand AC, Salminen S. Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli. J Med Microbiol 2003; 52:925–930 [View Article][PubMed]
    [Google Scholar]
  18. Orlando A, Linsalata M, Russo F. Antiproliferative effects on colon adenocarcinoma cells induced by co-administration of vitamin K1 and Lactobacillus rhamnosus GG . Int J Oncol 2016; 48:2629–2638 [View Article][PubMed]
    [Google Scholar]
  19. Pourshafie MR, Marklund BI, Ohlson S. Binding interactions of Escherichia coli with globotetraosylceramide (globoside) using a surface plasmon resonance biosensor. J Microbiol Methods 2004; 58:313–320 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.000591
Loading
/content/journal/jmm/10.1099/jmm.0.000591
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error