1887

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Volume 67, Issue 3

Molecular epidemiology of in Greece, 2010–2015 Open Access

Abstract

To determine the predominant strains of in Greece during 2010–2015.

Infants and children (=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS/IS real-time PCR confirmed spp./ infection in 300 samples. A subset of samples (=153) were analysed by multi-locus variable number tandem repeat analysis (MLVA) and (=25) by sequence-based typing of the toxin promotor region () on DNA extracted from clinical specimens.

A complete MLVA profile was determined in 66 out of 153 samples; the MLVA type 27 (=55) was the dominant genotype and all tested samples (=25) expressed the genotype. The vaccine coverage in the Greek population was 90 %; however, the study population expressed complete coverage in 2 out of 264 infants (0–11 months) and in 20 out of 36 children (1–14 years). Roma and immigrant minorities represent 7 % of the Greek population, but make up 50 % of the study population, indicating a low vaccine coverage among these groups.

The MT27 and genotype is dominant in Greek, Roma and immigrant infants and children hospitalized in Greece. Thus, the predominant MLVA genotype in Greece is similar to other countries using acellular vaccines.

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  • Accepted:
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Keyword(s): Bordetella pertussis , Greece , molecular typing , multi-Locus variable number tandem repeat analysis (MLVA) , sequence-based typing and whooping cough
© 2018 The Authors
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2018-03-01
2024-04-20
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Molecular epidemiology of Bordetella pertussis in Greece, 2010–2015
J. Med. Microbiol. 67, 400 (2018); https://doi.org/10.1099/jmm.0.000688
/content/journal/jmm/10.1099/jmm.0.000688
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