The inhibition of Caco-2 proliferation by astaxanthin from Xanthophyllomyces dendrorhous
Wayakanon, Kornchanok and Rueangyotchanthana, Kanjana and Wayakanon, Praween and Suwannachart, Chatrudee,, 67, 507-513 (2018),
doi = https://doi.org/10.1099/jmm.0.000710,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= Purpose. To investigate the efficiency of natural astaxanthin that has been extracted from Xanthophyllomyces dendrorhous in inhibiting the proliferation and viability of colorectal adenocarcinoma cell line (Caco-2; colon cancer cells). Methodology. Caco-2 cells and normal human oralkeratinocytes (NOKs) were treated with different concentrations of extracted astaxanthin, ranging from 0.075 to 10 mg ml−1, for 24, 48 and 72 h. The number of cells was determined via MTS assay and the proliferating cells were investigated by bromodeoxyuridine (BrdU) assay. Results/Key findings. Of the Caco-2 cells, 30–50 % remained viable, while the NOKs showed 110–120 % survival when treated with 5 mg ml−1 astaxanthin. The Caco-2 cells showed distinct structural shrinkage when treated with the same concentration of astaxanthin. Fluorescent labelling of the DNA of the proliferative cells with BrdU showed a significant decrease in the number of the proliferative Caco-2 cells when the concentration of astaxanthin was increased to 5 mg ml−1. Conclusion. The natural astaxanthin from X. dendrorhous, at an appropriate concentration, is effective in terminating the viability of, or retarding the proliferative activity of, Caco-2 cells, without harmful effects on NOKs.,
language=,
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