@article{mbs:/content/journal/jmm/10.1099/jmm.0.000730, author = "Zowawi, Hosam M. and Syrmis, Melanie W. and Kidd, Timothy J. and Balkhy, Hanan H. and Walsh, Timothy R. and Al Johani, Sameera M. and Al Jindan, Reem Y. and Alfaresi, Mubarak and Ibrahim, Emad and Al-Jardani, Amina and Al Salman, Jameela and Dashti, Ali A. and Sidjabat, Hanna E. and Baz, Omar and Trembizki, Ella and Whiley, David M. and Paterson, David L.", title = "Identification of carbapenem-resistant Pseudomonas aeruginosa in selected hospitals of the Gulf Cooperation Council States: dominance of high-risk clones in the region", journal= "Journal of Medical Microbiology", year = "2018", volume = "67", number = "6", pages = "846-853", doi = "https://doi.org/10.1099/jmm.0.000730", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000730", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "Saudi Arabia", keywords = "Gulf", keywords = "resistant", keywords = "Pseudomonas aeruginosa", keywords = "carbapenem", abstract = " Purpose. The molecular epidemiology and resistance mechanisms of carbapenem-resistant Pseudomonas aeruginosa (CRPA) were determined in hospitals in the countries of the Gulf Cooperation Council (GCC), namely, Saudi Arabia, the United Arab Emirates, Oman, Qatar, Bahrain and Kuwait. Methodology. Isolates were screened for common carbapenem-resistance genes by PCR. Relatedness between isolates was assessed using previously described genotyping methods: an informative-single nucleotide polymorphism MassARRAY iPLEX assay (iPLEX20SNP) and the enterobacterial repetitive intergenic consensus (ERIC)-PCR assay, with selected isolates being subjected to multilocus sequence typing (MLST). Ninety-five non-repetitive isolates that were found to be resistant to carbapenems were subjected to further investigation. Results/Key findings. The most prevalent carbapenemase-encoding gene, bla VIM-type, was found in 37/95 (39 %) isolates, while only 1 isolate (from UAE) was found to have bla IMP-type. None of the CRPA were found to have bla NDM-type or bla KPC-type. We found a total of 14 sequence type (ST) clusters, with 4 of these clusters being observed in more than 1 country. Several clusters belonged to the previously recognized internationally disseminated high-risk clones ST357, ST235, ST111, ST233 and ST654. We also found the less predominant ST316, ST308 and ST823 clones, and novel MLST types (ST2010, ST2011, ST2012 and ST2013), in our collection. Conclusion. Overall our data show that ‘high-risk’ CRPA clones are now detected in the region and highlight the need for strategies to limit further spread of such organisms, including enhanced surveillance, infection control precautions and further promotion of antibiotic stewardship programmes.", }