1887

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Volume 67, Issue 6

The BceABRS four-component system that is essential for cell envelope stress response is involved in sensing and response to host defence peptides and is required for the biofilm formation and fitness of Free

Abstract

is a primary cariogenic pathogen worldwide. In dental biofilms, often faces life-threatening insults, such as killing by antimicrobial compounds from competing species and from the host. How such insults affect the physiology and virulence of is poorly understood. In this study, we explored this question by investigating the responses of strains to several host defence peptides and bacitracin.

UA159 and its isogenic mutants, SmΔbceA, SmΔbceB, SmΔbceR and SmΔbceS, were examined for their antibiotic susceptibility and biofilm formation. The reporter strains were constructed to assay the responses of to host defence peptides. In addition, the competitive fitness of these mutants against the parent in response to peptide antibiotics was determined in dual-strain mixed cultures.

UA159 (WT) was generally insensitive to physiological concentrations of α-defensin-1, β-defensin-3, LL-37 and histatin-5, but all of the BceABRS mutants were sensitive to these peptide antibiotics. The response of to these peptide antibiotics involved the transcriptional activation of the operon itself. Bacitracin or β-defensin-3 at a sub-inhibitory concentration induced biofilm formation in the parent, but not in any of the BceABRS mutants. None of the mutants were able to compete with the parent for persistence in duel-strain cultures in the presence of bacitracin or β-defensin-3.

The BceABRS four-component system in is involved in sensing, response and resistance to host defence peptides, and is required for the biofilm formation and fitness of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibiotic sensing and response , biofilm formation , ecological fitness , host defense peptides , Streptococcus mutans and virulence
© 2018 The Authors
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2018-06-01
2024-04-25
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The BceABRS four-component system that is essential for cell envelope stress response is involved in sensing and response to host defence peptides and is required for the biofilm formation and fitness of Streptococcus mutans
J. Med. Microbiol. 67, 874 (2018); https://doi.org/10.1099/jmm.0.000733
/content/journal/jmm/10.1099/jmm.0.000733
/content/journal/jmm/10.1099/jmm.0.000733
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