RT Journal Article SR Electronic(1) A1 Tasca Ribeiro, Victoria Stadler A1 Tuon, Felipe Francisco A1 Kraft, Letícia A1 Suss, Paula Hansen A1 Wollmann, Luciana Cristina A1 Roderjan, João Gabriel A1 Brito, Diego Armando A1 Alexandrino, Fabiana A1 Malgarin, Juliane Soldi A1 Morello, Luis Gustavo A1 da Costa, Francisco Diniz Affonso A1 Pillonetto, MarceloYR 2018 T1 Conventional culture method and qPCR using 16S rDNA for tissue bank: a comparison using a model of cardiac tissue contamination JF Journal of Medical Microbiology, VO 67 IS 11 SP 1571 OP 1575 DO https://doi.org/10.1099/jmm.0.000837 PB Microbiology Society, SN 1473-5644, AB Real-time polymerase chain reaction (qPCR) using 16S rDNA is an alternative to conventional culture-based tests. The aim of this study was to compare the conventional culture method with qPCR using 16S rDNA in a model of cardiac tissue contamination. Samples of cardiac tissue for artificial contamination with Escherichia coli and control samples were submitted for DNA extraction, which was conducted by selective and alkaline lysis and purification steps. A standard curve for 16S rDNA was constructed to determine the efficiency and analytical sensitivity of the assay in concentrations from 106 to 102 c.f.u. ml−1 using TaqMan Master Mix. 16S rDNA was detected in all contaminated samples; however, it was not detected in the the final washing step solution of the sample with a bioburden of 102 c.f.u. ml−1. Using qPCR is a potential alternative to conventional culture for microbiological safety testing of allograft tissues for biobanking, reducing the time and labour input required., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000837