%0 Journal Article %A Vanstone, G. L. %A Wey, E. %A Mack, D. %A Smith, E. R. %A Balakrishnan, I. %T Evaluation of the EntericBio CPE assay for the detection of carbapenemase-producing organisms %D 2018 %J Journal of Medical Microbiology, %V 67 %N 12 %P 1728-1730 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.000851 %K carbapenemase %K resistance %K detection %K molecular %I Microbiology Society, %X Carbapenemase-producing organisms (CPOs) represent an increasing threat in healthcare facilities and detection of these organisms in the diagnostic laboratory can be challenging. The EntericBio CPE (EBCPE) real-time PCR assay (Serosep Ltd) was evaluated for the detection of NDM, KPC, OXA-48-like, VIM, IMP and GES carbapenemase genes from a panel of 145 multidrug-resistant organisms (29 NDM, 35 OXA-48, 21 VIM, 4 OXA-23, 3 KPC, 5 NDM+OXA-48, 3 GES-5, 1 OXA-23+NDM, 1 IMI, 2 IMP-1 and 41 multidrug-resistant carbapenemase-negative isolates). The EBCPE assay performed well, with 100 % sensitivity and specificity for the detection of all genotypes included in the assay. Turnaround time and laboratory workflow were improved compared to culture-based assays. Users must remain aware of the limitations of molecular assays for CPO detection to ensure implementation of the most suitable CPO diagnostic pathways. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.000851