Binding of Pneumocystis carinii to the lung epithelial cell receptor HSPA5 (GRP78)
Kottom, Theodore J. and Hebrink, Deanne M. and Limper, Andrew H.,, 67, 1772-1777 (2018),
doi = https://doi.org/10.1099/jmm.0.000864,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= The importance of lung macrophages in Pneumocystis–host interaction is well known, but little is known about the initial binding/colonization of the airway epithelium. Our prior studies have documented cell-signalling events that occur following binding of the organisms to lung epithelial cells; however, the receptors that mediate Pneumocystis attachment to lung surfaces have not yet been fully defined. Using affinity chromatography, we identified heat shock protein 5 (HSPA5), also known as GRP78, as a potential host receptor that may have relevance in Pneumocystis lung colonization. Pneumocystis carinii (Pc) organisms not only bound HSPA5 on a rat lung epithelial cell line, but also on primary rat airway epithelial cells (AECs). Furthermore, Pc bound CHO1 cells overexpressing HSPA5 more than the CHO1 parent line alone, supporting a role for Pc–HSPA5 protein interaction in mediating organism attachment. These results provide new insights into the interactions of Pneumocystis with host lung epithelium.,
language=,
type=