1887

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Volume 73, Issue 4

Evaluation of a new CT/NG/TV/MG Real-Time PCR Kit (Vircell) versus the Allplex STI Essential Assay (Seegene) for the diagnosis of sexually transmitted infections No Access

Abstract

Sexually transmitted infections (STI) are a public health problem. Real-time PCR assays are the most sensitive test for screening and diagnosis of these infections. The aim of this study was to evaluate a new CT/NG/TV/MG Real-Time PCR (RT-PCR) kit (Vircell) for the detection of , , and for the diagnosis of sexual transmitted infections using the Allplex STI Essential Assay (Seegene) as the reference’s method. A total of 497 samples from different anatomical sites (endocervical, urethral, rectal, pharyngeal and urine) were analysed from October 2022 to February 2023. A total of 108 (21.73 %) and 106 (21.33 %) positive samples were found for any of the assays used. The most commonly detected pathogen was (52 samples; 10.46 %), and the least commonly detected was (three samples; 0.60 %). The anatomical site with the highest prevalence of micro-organisms was a non-urogenital site, the pharynx (26 positive samples; 5.23 %). Using the Allplex STI Essential Assay (Seegene) as the reference method, the diagnosis performance showed that the average specificity of CT/NG/TV/MG RT-PCR Kit (Vircell) was 99.84 % and the sensitivity was 99.53 %. The overall concordance was k=0.98 (CI95 %; 0.96–1). In conclusion, the CT/NG/TV/MG RT-PCR Kit (Vircell) assay shows a good sensitivity and specificity and constitutes a promising and additional alternative to routine procedures for distinct types of clinical specimen in diagnosis STI.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Chlamydia trachomatis , diagnosis , Mycoplasma genitalium , Neisseria gonorrhoeae , real time-PCR , sexually transmitted infections and Trichomonas vaginalis
© 2024 The Authors
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/content/journal/jmm/10.1099/jmm.0.001797
2024-04-09
2024-04-30
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Evaluation of a new CT/NG/TV/MG Real-Time PCR Kit (Vircell) versus the Allplex STI Essential Assay (Seegene) for the diagnosis of sexually transmitted infections
J. Med. Microbiol. 73, 001797 (2024); https://doi.org/10.1099/jmm.0.001797
/content/journal/jmm/10.1099/jmm.0.001797
/content/journal/jmm/10.1099/jmm.0.001797
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