RT Journal Article SR Electronic(1) A1 Loeffler, Juergen A1 Haddad, Ziad A1 Bonin, Michael A1 Romeike, Nele A1 Mezger, Markus A1 Schumacher, Ulrike A1 Kapp, Markus A1 Gebhardt, Florian A1 Grigoleit, Goetz-Ulrich A1 Stevanović, Stefan A1 Einsele, Hermann A1 Hebart, HolgerYR 2009 T1 Interaction analyses of human monocytes co-cultured with different forms of Aspergillus fumigatus JF Journal of Medical Microbiology, VO 58 IS 1 SP 49 OP 58 DO https://doi.org/10.1099/jmm.0.003293-0 PB Microbiology Society, SN 1473-5644, AB Monocytes play a major role in the cellular defence against Aspergillus fumigatus in immunocompromised patients. To obtain a better understanding of the mechanisms involved in this interaction, phagocytosis and gene expression profiling of human monocytes was carried out after incubation with A. fumigatus resting, swollen and germinating conidia and hyphae (for 3, 6 and 9 h). The majority of monocytes phagocytosed up to three conidia during the first 3 h of incubation. Microarray analysis showed an increased expression level of immune-relevant genes, which was dependent on the germination state of the fungus and the incubation period. Among these genes, those encoding interleukin-8, macrophage inflammatory protein 3-α (CCL20) and monocyte chemotactic protein-1 (CCL2) were found to be potential key regulators involved in the A. fumigatus-induced immune response. In addition, A. fumigatus was found to be an inducer of the genes encoding urokinase type plasminogen activator (uPA), urokinase type plasminogen activator receptor (uPAR),plasminogen activator inhibitor (PAI), pentraxin-3 (PTX3) and intercellular adhesion molecule-1 (ICAM-1), which, in combination, may contribute to thrombosis and local lung tissue injury., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.003293-0