1887

Abstract

The prevalence of endocarditis (AE) is increasing in the hospital population. species contribute to approximately 25 % of all cases of fungal endocarditis. This study is a descriptive report of the use of nested PCR to detect DNA specific for pecies in serum for the diagnosis of cardiac infections. Open heart surgery was performed on patients and collected samples were examined microscopically and cultured. Ten sera in total from five patients were extracted for DNA and nested PCR with species primers was carried out. The lowest limit of detection for the PCR assay was 1 c.f.u. (ml serum) . The PCR was positive in three patients. Culture of valvular tissue confirmed the growth of in one patient and in two patients. In this study we have demonstrated the presence of invasive aspergillosis in patients who had undergone open heart surgery and the usefulness of a molecular assay for the diagnosis of AE.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.004531-0
2009-02-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/2/192.html?itemId=/content/journal/jmm/10.1099/jmm.0.004531-0&mimeType=html&fmt=ahah

References

  1. Anaissie E. J., McGinnis M. R., Pfaller M. A. 2003 Clinical Mycology p 286 New York & Philadelphia, PA: Churchill Livingstone;
    [Google Scholar]
  2. Aslam P. A., Gourley R., Eastridge C. E., Pate J. W. 1970; Aspergillus endocarditis after aortic valve replacement. Int Surg 53:91–96
    [Google Scholar]
  3. Badiee P., Kordbacheh P., Alborzi A., Malekhoseini S. A., Zeini F., Mirhendi H., Mahmoodi M. 2007; Prospective screening in liver transplant recipients by panfungal PCR-ELISA for early diagnosis of invasive fungal infections. Liver Transpl 13:1011–1016 [CrossRef]
    [Google Scholar]
  4. Breitkopf C., Hammel D., Scheld H. H., Peters G., Becker K. 2005; Impact of a molecular approach to improve the microbiological diagnosis of infective heart valve endocarditis. Circulation 111:1415–1421 [CrossRef]
    [Google Scholar]
  5. Durack D. T., Lukes A. S., Bright D. K. 1994; New criteria for the diagnosis of infective endocarditis: utilization of specific endocardiographic findings: Duke endocarditis service. Am J Med 96:200–209 [CrossRef]
    [Google Scholar]
  6. Einsele H., Hebart H., Roller G., Löffler J., Rothenhofer I., Müller C. A., Bowden R. A., Van Burik J., Engelhard D. other authors 1997; Detection and identification of fungal pathogens in blood by using molecular probes. J Clin Microbiol 35:1353–1360
    [Google Scholar]
  7. El-Hamamsy I., Dürrleman N., Stevens L. M., Cartier R., Pellerin M., Perrault L. P., Carrier M. 2004; A cluster of cases of Aspergillus endocarditis after cardiac surgery. Ann Thorac Surg 77:2184–2186 [CrossRef]
    [Google Scholar]
  8. Ellis M. E., Al-Abdely H., Sandridge A., Greer W., Ventura W. 2001; Fungal endocarditis: evidence in the world literature, 1965–1995. Clin Infect Dis 32:50–62 [CrossRef]
    [Google Scholar]
  9. Feigin R. D., Cherry J. D., Demmler G. J., Kaplan S. L. 2004 Textbook of Pediatric Infectious Diseases , 5th edn. vol 2 Philadelphia, PA: Saunders;
    [Google Scholar]
  10. Gerritsen J. G., Van Dissel J. T., Verwey H. F. 1998; Candida tropicalis endocarditis. Circulation 98:90–91 [CrossRef]
    [Google Scholar]
  11. Gumbo T., Taege A. J., Mawhorter S., McHenry M. C., Lytle B. H., Congrove D. M., Gordon S. M. 2000; Aspergillus valve endocarditis in patients without prior cardiac surgery. Medicine 79:261–268 [CrossRef]
    [Google Scholar]
  12. Imai T., Sano A., Mikami Y., Watanabe K., Aoke F. H., Branchini M. L. M., Negroni R., Nishimura K., Miyaji M. 2000; A new PCR primer for the identification of Paracoccidioides brasiliensis based on rRNA sequences coding the internal transcribed spacers (ITS) and 5.8S regions. Med Mycol 38:323–326 [CrossRef]
    [Google Scholar]
  13. Khan T. H., Kane E. G., Dean D. C. 1968; Aspergillus endocarditis of mitral prosthesis. Am J Cardiol 22:277–280 [CrossRef]
    [Google Scholar]
  14. Kotanidou A. N., Zakynthinos E., Andrianakis I., Zervakis D., Kokotsakis I., Argyrakos T., Argiropoulou A., Margariti G., Douzinas E. 2004; Aspergillus endocarditis in a native valve after amphotericin B treatment. Ann Thorac Surg 78:1453–1455 [CrossRef]
    [Google Scholar]
  15. Löffler J., Hebart H., Sepe S., Schumcher U., Klingebiel T., Einsele H. 1998; Detection of PCR-amplified fungal DNA by using PCR-ELISA system. Med Mycol 36:275–279 [CrossRef]
    [Google Scholar]
  16. McCracken D., Barnes R., Poynton C., White P. L., Iik N., Cook D. 2003; Polymerase chain reaction aids in the diagnosis of an unusual case of Aspergillus niger endocarditis in a patient with acute myeloid leukaemia. J Infect 47:344–347 [CrossRef]
    [Google Scholar]
  17. Melgar G. R., Nasser R. M., Gordon S. M., Lytle B. W., Keys T. F., Longworth D. L. 1997; Fungal prosthetic valve endocarditis in 16 patients: an 11-year experience in a tertiary care hospital. Medicine 76:94–103 [CrossRef]
    [Google Scholar]
  18. Muehrcke D. D., Lytle B. W., Cosgrove D. M. 1995; Surgical and long term antifungal therapy for fungal prosthetic valve endocarditis. Ann Thorac Surg 60:538–543 [CrossRef]
    [Google Scholar]
  19. Pemán J., Ortiz R., Osseyran F., Pérez-Bellés C., Crespo M., Chirivella M., Frasquet J., Quesada A., Cantón E., Gobernado M. 2007; Native valve Aspergillus fumigatus endocarditis with blood culture positive and negative for galactomannan antigen. Case report and literature review. Rev Iberoam Micol 24:157–160 [CrossRef]
    [Google Scholar]
  20. Posteraro B. M., Sanguietti L., Masucci L., Romano G., Morace J., Fadda G. 2000; Reverse cross blot hybridization assay for rapid detection of PCR-amplified DNA from Candida species Cryptococous neoformans and Saccharomyces cerevisiae in clinical samples. J Clin Microbiol 38:1609–1614
    [Google Scholar]
  21. Puvimanasinghe J. P. E., Steyerberg E. W., Takkenberg J. J. M. 2001; Prognosis after aortic valve replacement with a bioprosthesis: predictions based on meta-analysis and microsimulation. Circulation 103:1535–1541 [CrossRef]
    [Google Scholar]
  22. Rubinstein E., Lang R. 1995; Fungal endocarditis. Eur Heart J 16:84–89
    [Google Scholar]
  23. Sherman-Weber S., Axelrod P., Suh B., Rubin S., Beltramo D., Manacchio J., Furukawa S., Weber T., Eisen H., Samuel R. 2004; Infective endocarditis following orthotopic heart transplantation: 10 cases and a review of the literature. Transpl Infect Dis 6:165–170 [CrossRef]
    [Google Scholar]
  24. Stein P. D., Harken D. E., Dexter L. 1966; The nature and prevention of prosthetic valve endocarditis. Am Heart J 71:393–407 [CrossRef]
    [Google Scholar]
  25. Van Burik J. A., Myerson D., Schreckhise R. W., Bowden R. A. 1998; Panfungal PCR assay for detection of fungal infection in human blood specimens. J Clin Microbiol 36:1169–1175
    [Google Scholar]
  26. Yamakami Y., Hashimoto A., Tokimatsu I., Nasu M. 1996; PCR detection of DNA specific for Aspergillus species in serum of patients with invasive aspergillosis. J Clin Microbiol 34:2464–2468
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.004531-0
Loading
/content/journal/jmm/10.1099/jmm.0.004531-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error