1887

Abstract

is an anaerobic spirochaete that colonizes the large intestine of humans and various species of animals and birds. The spirochaete is an important enteric pathogen of pigs and poultry, but its pathogenic potential in humans is less clear. In the current study, the occurrence of in faecal samples from 766 individuals in two different population groups in Perth, Western Australia, was investigated by selective anaerobic culture. Of 586 individuals who were long-term residents of Perth, including children, elderly patients in care and in hospital and individuals with gastrointestinal disease, only one was culture positive. This person had a history of diverticulitis. In comparison, faeces from 17 of 180 (9.4 %) Indonesians who were short- or medium-term visitors to Perth were positive for . The culture-positive individuals had been in the city for between 10 days and 4.5 years (median 5 months). Resampling of subsets of the Indonesians indicated that all negative people remained negative and that some positive individuals remained positive after 5 months. Two individuals had pairs of isolates recovered after 4 and 5 months that had the same PFGE types, whilst another individual had isolates with two different PFGE types that were identified 2 months apart. Individuals who were culture-positive were likely to have been either colonized in Indonesia before arriving in Perth or infected in Perth following contact with other culture-positive Indonesians with whom they socialized. Colonization with was not significantly associated with clinical signs at the time the individuals were tested, although faeces with wet-clay consistency were 1.5 times more likely (confidence interval 0.55–4.6) than normal faeces to contain .

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2009-02-01
2024-03-29
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References

  1. Barrett S. P. 1990; Intestinal spirochaetes in a Gulf Arab population. Epidemiol Infect 104:261–266 [CrossRef]
    [Google Scholar]
  2. Brooke C. J., Clair A. N., Mikosza A. S. J., Riley T. V., Hampson D. J. 2001; Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia. Epidemiol Infect 127:369–374
    [Google Scholar]
  3. Brooke C. J., Riley T. V., Hampson D. J. 2006; Comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in four Australian populations. Epidemiol Infect 134:627–634 [CrossRef]
    [Google Scholar]
  4. Fournié-Amazouz E., Baranton G., Carlier J. P., Chambreuil G., Cohadon F., Collin P., Jolivet A. G., Hermès I., Lemarie C., Saint Girons I. 1995; Isolations of intestinal spirochaetes from the blood of human patients. J Hosp Infect 30:160–162 [CrossRef]
    [Google Scholar]
  5. Goossens H., Lejour M., de Mol P., de Boeck M., Verhaeghen G., Dekegel D., Butzler J. P. 1983; Isolation of Treponema hyodysenteriae from human faecal specimens: a curiosity or a new enteropathogen?. In Proceedings of the European Congress of Clinical Microbiology 1983 p 297 Bologna, Italy:
    [Google Scholar]
  6. Hampson D. J., Duhamel G. E. 2006; Porcine colonic spirochetosis/intestinal spirochetosis. In Diseases of Swine , 9th edn. pp 755–767 Edited by Straw B. E., Zimmerman J. J., D'Allaire S., Taylor D. J. Oxford: Blackwell Publishing;
    [Google Scholar]
  7. Hampson D. J., Swayne D. E. 2008; Avian intestinal spirochetosis. In Diseases of Poultry , 12th edn. pp 826–835 Edited by Saif Y. M. Oxford: Blackwell Publishing;
    [Google Scholar]
  8. Hampson D. J., Oxberry S. L., La T. 2006; Potential for zoonotic transmission of Brachyspira pilosicoli . Emerg Infect Dis 12:869–870 [CrossRef]
    [Google Scholar]
  9. Jenkinson S. R., Wingar C. R. 1981; Selective medium for the isolation of Treponema hyodysenteriae . Vet Rec 109:384–385 [CrossRef]
    [Google Scholar]
  10. Kanavaki S., Mantadakis E., Thomakos N., Pefanis A., Matsiota-Bernard P., Karabela S., Samonis G. 2002; Brachyspira ( Serpulina ) pilosicoli spirochetemia in an immunocompromised patient. Infection 30:175–177 [CrossRef]
    [Google Scholar]
  11. Käsbohrer A., Gelderblom H., Arasteh K., Heise W., Grosse G., L'age M., Schönberg A., Koch M.A., Pauli G. 1990; Intestinal spirochetosis in HIV infection: prevalence, isolation and morphology of spirochetes. Dtsch Med Wochenschr 115:1499–1506 (in German [CrossRef]
    [Google Scholar]
  12. Kunkle R. A., Harris D. L., Kinyon J. M. 1986; Autoclaved liquid medium for propagation of Treponema hyodysenteriae . J Clin Microbiol 24:669–671
    [Google Scholar]
  13. Lee J. I., Hampson D. J. 1992; Intestinal spirochaetes colonizing Aborigines from communities in the remote north of Western Australia. Epidemiol Infect 109:133–141
    [Google Scholar]
  14. Lee J. I., Hampson D. J. 1994; Genetic characterisation of intestinal spirochaetes and their association with disease. J Med Microbiol 40:365–371 [CrossRef]
    [Google Scholar]
  15. Lima M. A., Barbosa A. L., Santos V. M., Misiara F. P. 2005; Intestinal spirochetosis and colon diverticulosis. Rev Soc Bras Med Trop 38:56–57
    [Google Scholar]
  16. Margawani K. R., Robertson I. D., Brooke C. J., Hampson D. J. 2004; Prevalence, risk factors and molecular epidemiology of Brachyspira pilosicoli in humans on the island of Bali. Indonesia. J Med Microbiol 53:325–332 [CrossRef]
    [Google Scholar]
  17. Mikosza A. S. J., Hampson D. J. 2001; Human intestinal spirochetosis: Brachyspira aalborgi and/or Brachyspira pilosicoli ?. Anim Health Res Rev 2:101–110
    [Google Scholar]
  18. Mikosza A. S. J., La T., Brooke C. J., Lindboe C. F., Ward P. B., Heine R. G., Guccion J. G., de Boer W. B., Hampson D. J. 1999; Polymerase chain reaction amplification from fixed tissue indicates the frequent involvement of Brachyspira aalborgi in human intestinal spirochetosis. J Clin Microbiol 37:2093–2098
    [Google Scholar]
  19. Munshi M. A., Traub R. J., Robertson I. D., Mikosza A. S. J., Hampson D. J. 2004; Colonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea-estates in Assam, India. Epidemiol Infect 132:137–144 [CrossRef]
    [Google Scholar]
  20. Oxberry S. L., Trott D. J., Hampson D. J. 1998; Serpulina pilosicoli , water birds and water: potential sources of infection for humans and other animals. Epidemiol Infect 121:219–225 [CrossRef]
    [Google Scholar]
  21. Tompkins D. S., Foukes S. J., Godwin P. G. R., West A. P. 1986; Isolation and characterization of intestinal spirochaetes. J Clin Pathol 39:535–541 [CrossRef]
    [Google Scholar]
  22. Trivett-Moore N. L., Gilbert G. L., Law C. L. H., Trott D. J., Hampson D. J. 1998; Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. J Clin Microbiol 36:261–265
    [Google Scholar]
  23. Trott D. J., Combs B. G., Oxberry S. L., Mikosza A. S. J., Robertson I. D., Passey M., Taime J., Sehuko R., Hampson D. J. 1997a; The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea. Epidemiol Infect 119:369–379 [CrossRef]
    [Google Scholar]
  24. Trott D. J., Jensen N. S., Saint Girons I., Oxberry S. L., Stanton T. B., Lindquist D., Hampson D. J. 1997b; Identification and characterisation of Serpulina pilosicoli isolates from the blood of critically-ill patients. J Clin Microbiol 35:482–485
    [Google Scholar]
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