1887

Abstract

We screened 500 pregnant women who had no risk factors for vaginal carriage, and isolated 39 strains (8 %). The density of carriage was low in 16 cases (41 %), intermediate in 16 cases (41 %) and heavy in seven cases (18 %). Strains were mostly of serotype III (41 %), Ia (26 %) and V (18 %). Thirty-five strains had at least one of five genetic markers that have been associated with virulent phylogenetic subgroups of strains. Using PCR, nine strains (23 %) were identified as belonging to CC17. The 39 vaginal strains that were studied exhibited a substantial genetic diversity; there were 39 PFGE profiles and 13 variants defined on the basis of the five genetic markers studied. The prevalence of the studied genetic characteristics was similar for strains associated with all three classes of density of carriage. These data suggest that genetic features that are markers of strains able to invade the central nervous system of neonates are not determinants for vaginal adaptation.

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2009-02-01
2024-03-28
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References

  1. Agence Nationale d'Accréditation et d'Évaluation en Santé 2001; Antenatal prevention of the risk of early neonatal bacterial infection. [Online] http://www.has-sante.fr/portail/upload/docs/application/pdf/Antenatal_prevention.pdf
  2. Anthony B. F., Okada D. M. 1977; The emergence of group B streptococci in infections of the newborn infant. Annu Rev Med 28:355–369 [CrossRef]
    [Google Scholar]
  3. Bidet P., Brahimi N., Chalas C., Aujard Y., Bingen E. 2003; Molecular characterization of serotype III group B- Streptococcus isolates causing neonatal meningitis. J Infect Dis 188:1132–1137 [CrossRef]
    [Google Scholar]
  4. Bohnsack J. F., Whiting A., Gottschalk M., Dunn D. M., Weiss R., Azimi P. H., Philips J. B. III, Weisman L. E., Rhoads G. G., Lin F.-Y. C. 2008; Population structure of invasive and colonizing strains of Streptococcus agalactiae from neonates of six U.S. academic centers from 1995 to 1999. J Clin Microbiol 46:1285–1291 [CrossRef]
    [Google Scholar]
  5. Granlund M., Michel F., Norgren M. 2001; Mutually exclusive distribution of IS 1548 and GBSi1, an active group II intron identified in human isolates of group B streptococci. J Bacteriol 183:2560–2569 [CrossRef]
    [Google Scholar]
  6. Jones N., Bohnsack J. F., Takahashi S., Oliver K. A., Chan M. S., Kunst F., Glaser P., Rusniok C., Crook D. W. other authors 2003; Multilocus sequence typing system for group B Streptococcus . J Clin Microbiol 41:2530–2536 [CrossRef]
    [Google Scholar]
  7. Kong F., Gowan S., Martin D., James G., Gilbert G. L. 2002; Serotype identification of group B streptococci by PCR and sequencing. J Clin Microbiol 40:216–226 [CrossRef]
    [Google Scholar]
  8. Lamy M. C., Dramsi S., Billoet A., Reglier-Poupet H., Tazi A., Raymond J., Guerin F., Couve E., Kunst F. other authors 2006; Rapid detection of the “highly virulent” group B Streptococcus ST-17 clone. Microbes Infect 8:1714–1722 [CrossRef]
    [Google Scholar]
  9. Lin F.-Y. C., Whiting A., Adderson E., Takahashi S., Dunn D. M., Weiss R., Azimi P. H., Philips J. B. III, Weisman L. E. other authors 2006; Phylogenetic lineages of invasive and colonizing strains of serotype III group B streptococci from neonates: a multicenter prospective study. J Clin Microbiol 44:1257–1261 [CrossRef]
    [Google Scholar]
  10. Musser J. M., Mattingly S. J., Quentin R., Goudeau A., Selander R. K. 1989; Identification of a high-virulence clone of type III Streptococcus agalactiae (group B Streptococcus ) causing invasive neonatal disease. Proc Natl Acad Sci U S A 86:4731–4735 [CrossRef]
    [Google Scholar]
  11. Quentin R., Huet H., Wang F. S., Geslin P., Goudeau A., Selander R. K. 1995; Characterization of Streptococcus agalactiae strains by multilocus enzyme genotype and serotype: identification of multiple virulent clone families that cause invasive neonatal disease. J Clin Microbiol 33:2576–2581
    [Google Scholar]
  12. Rallu F., Barriga P., Scrivo C., Martel-Laferrière V., Laferrière C. 2006; Sensitivities of antigen detection and PCR assays greatly increased compared to that of the standard culture method for screening for group B Streptococcus carriage in pregnant women. J Clin Microbiol 44:725–728 [CrossRef]
    [Google Scholar]
  13. Rolland K., Marois C., Siquier V., Cattier B., Quentin R. 1999; Genetic features of Streptococcus agalactiae strains causing severe neonatal infections, as revealed by pulsed-field gel electrophoresis and hylB gene analysis. J Clin Microbiol 37:1892–1898
    [Google Scholar]
  14. Schrag S., Gorwitz R., Fultz-Butts K., Schuchat A. 2002; Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC. MMWR Recomm Rep 51:1–22
    [Google Scholar]
  15. Takahashi S., Adderson E. E., Nagano Y., Nagano N., Briesacher M. R., Bohnsack J. F. 1998; Identification of a highly encapsulated, genetically related group of invasive type III group B streptococci. J Infect Dis 177:1116–1119 [CrossRef]
    [Google Scholar]
  16. van der Mee-Marquet N., Domelier A. S., Mereghetti L., Lanotte P., Rosenau A., van Leeuwen W., Quentin R. 2006; Prophagic DNA fragments in Streptococcus agalactiae strains and association with neonatal meningitis. J Clin Microbiol 44:1049–1058 [CrossRef]
    [Google Scholar]
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