@article{mbs:/content/journal/jmm/10.1099/jmm.0.006031-0, author = "Antonio, Martin and Hakeem, Ishrat and Sankareh, Kawsu and Cheung, Yin Bun and Adegbola, Richard A.", title = "Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions", journal= "Journal of Medical Microbiology", year = "2009", volume = "58", number = "3", pages = "296-302", doi = "https://doi.org/10.1099/jmm.0.006031-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.006031-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "Sequential multiplex PCR was evaluated for detection of multiple Streptococcus pneumoniae serotypes directly from nasopharyngeal secretions. A total of 279 nasopharyngeal swab samples were tested blindly. When limited to the 29 serotypes identifiable by the molecular method, the mean number of serotypes identified by the conventional latex/Quellung method was 0.85, which was significantly lower than that by the molecular method (P <0.0001). The multiplex PCR method identified significantly more serotypes than the latex/Quellung method if limited to the 29 serotypes (P=0.001 and P=0.014, respectively).", }