%0 Journal Article %A Antonio, Martin %A Hakeem, Ishrat %A Sankareh, Kawsu %A Cheung, Yin Bun %A Adegbola, Richard A. %T Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions %D 2009 %J Journal of Medical Microbiology, %V 58 %N 3 %P 296-302 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.006031-0 %I Microbiology Society, %X Sequential multiplex PCR was evaluated for detection of multiple Streptococcus pneumoniae serotypes directly from nasopharyngeal secretions. A total of 279 nasopharyngeal swab samples were tested blindly. When limited to the 29 serotypes identifiable by the molecular method, the mean number of serotypes identified by the conventional latex/Quellung method was 0.85, which was significantly lower than that by the molecular method (P <0.0001). The multiplex PCR method identified significantly more serotypes than the latex/Quellung method if limited to the 29 serotypes (P=0.001 and P=0.014, respectively). %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.006031-0