1887

Journal of Medical Microbiology logo

Volume 58, Issue 5

Multiplex PCR reveals a high rate of nasopharyngeal pneumococcal 7-valent conjugate vaccine serotypes co-colonizing indigenous Warao children in Venezuela Free

Abstract

Knowledge of co-colonization with multiple pneumococcal serotypes is becoming very important in the light of both serotype replacement and switching as a result of vaccination. Co-colonization has been reported to occur in up to 30 % of carriers, especially in populations with high carriage rates. For the determination of co-colonization, single colonies of nasopharyngeal specimens are serotyped with the Quellung method, a costly method with a low sensitivity. Here we explore the use of a multiplex PCR to identify simultaneous carriage of the capsular serotypes targeted by the 7-valent conjugate vaccine. We applied this multiplex PCR to 50 primary cultures from the nasopharyngeal swabs of healthy Warao Amerindian children, a population with a high pneumococcal carriage rate, most of them with vaccine serotypes, and we identified a second serotype in 20 % (=10) of the pneumococci carriers. These results were confirmed by detailed serotyping of multiple colonies isolated from the primary culture with the Quellung method. We conclude that the multiplex PCR is a sensitive, simple and cost-effective method for detecting multiple serotypes in nasopharyngeal cultures, and thus might be useful for the monitoring of pneumococcal colonization over time, especially in the surveillance of nasopharyngeal colonization after conjugate vaccination.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ACIP, Advisory Committee on Immunization Practices
SGM
Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.006726-0
2009-05-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/jmm/58/5/584.html?itemId=/content/journal/jmm/10.1099/jmm.0.006726-0&mimeType=html&fmt=ahah

References

  1. ACIP 2000; Preventing pneumococcal disease among infants and young children. Recommendations of the Advisory Committee on Immunization Practices (ACIP. MMWR Recomm Rep 49:1–38
     [Google Scholar]
  2. Barker J., Gratten M., Riley I., Lehmann D., Montgomery J., Kajoi M., Gratten H., Smith D., Marshall T. F., Alpers M. P. 1989; Pneumonia in children in the Eastern Highlands of Papua New Guinea: a bacteriologic study of patients selected by standard clinical criteria. J Infect Dis 159:348–352 [CrossRef]
     [Google Scholar]
  3. Bogaert D., Veenhoven R. H., Sluijter M., Sanders E. A., de Groot R., Hermans P. W. 2004; Colony blot assay: a useful method to detect multiple pneumococcal serotypes within clinical specimens. FEMS Immunol Med Microbiol 41:259–264 [CrossRef]
     [Google Scholar]
  4. Bronsdon M. A., O'Brien K. L., Facklam R. R., Whitney C. G., Schwartz B., Carlone G. M. 2004; Immunoblot method to detect Streptococcus pneumoniae and identify multiple serotypes from nasopharyngeal secretions. J Clin Microbiol 42:1596–1600 [CrossRef]
     [Google Scholar]
  5. Byington C. L., Samore M. H., Stoddard G. J., Barlow S., Daly J., Korgenski K., Firth S., Glover D., Jensen J. other authors 2005; Temporal trends of invasive disease due to Streptococcus pneumoniae among children in the intermountain west: emergence of nonvaccine serogroups. Clin Infect Dis 41:21–29 [CrossRef]
     [Google Scholar]
  6. Dawid S., Roche A. M., Weiser J. N. 2007; The blp bacteriocins of Streptococcus pneumoniae mediated intraspecies competition both in vitro and in vivo. Infect Immun 75:443–451 [CrossRef]
     [Google Scholar]
  7. Gratten M., Montgomery J., Gerega G., Gratten H., Siwi H., Poli A., Koki G. 1989; Multiple colonization of the upper respiratory tract of Papua New Guinea children with Haemophilus influenzae and Streptococcus pneumoniae . Southeast Asian J Trop Med Public Health 20:501–509
     [Google Scholar]
  8. Gratten M., Manning K., Dixon J., Morey F., Torzillo P., Hanna J., Erlich J., Asche V., Riley I. 1994; Upper airway carriage by Haemophilus influenzae and Streptococcus pneumoniae in Australian aboriginal children hospitalised with acute lower respiratory infection. Southeast Asian J Trop Med Public Health 25:123–131
     [Google Scholar]
  9. Gray B. M., Converse G. M. III, Dillon H. C. Jr 1980; Epidemiologic studies of Streptococcus pneumoniae in infants: acquisition, carriage, and infection during the first 24 months of life. J Infect Dis 142:923–933 [CrossRef]
     [Google Scholar]
  10. Hare K. M., Morris P., Smith-Vaughan H., Leach A. J. 2008; Random colony selection versus colony morphology for detection of multiple pneumococcal serotypes in nasopharyngeal swabs. Pediatr Infect Dis J 27:178–180
     [Google Scholar]
  11. Huang S. S., Platt R., Rifas-Shiman S. L., Pelton S. I., Goldmann D., Finkelstein J. A. 2005; Post-PCV7 changes in colonizing pneumococcal serotypes in 16 Massachusetts communities, 2001 and 2004. Pediatrics 116:e408–e413 [CrossRef]
     [Google Scholar]
  12. Huebner R. E., Dagan R., Porath N., Wasas A. D., Klugman K. P. 2000; Lack of utility of serotyping multiple colonies for detection of simultaneous nasopharyngeal carriage of different pneumococcal serotypes. Pediatr Infect Dis J 19:1017–1020 [CrossRef]
     [Google Scholar]
  13. Kong F., Brown M., Sabananthan A., Zeng X., Gilbert G. L. 2006; Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes. J Clin Microbiol 44:1887–1891 [CrossRef]
     [Google Scholar]
  14. Lloyd-Evans N., O'Dempsey T. J., Baldeh I., Secka O., Demba E., Todd J. E., McArdle T. F., Banya W. S., Greenwood B. M. 1996; Nasopharyngeal carriage of pneumococci in Gambian children and in their families. Pediatr Infect Dis J 15:866–871 [CrossRef]
     [Google Scholar]
  15. Montgomery J. M., Lehmann D., Smith T., Michael A., Joseph B., Lupiwa T., Coakley C., Spooner V., Best B. other authors 1990; Bacterial colonization of the upper respiratory tract and its association with acute lower respiratory tract infections in Highland children of Papua New Guinea. Rev Infect Dis 12 (Suppl. 8):S1006–S1016 [CrossRef]
     [Google Scholar]
  16. Neufeld F. 1902; Uber die Agglutination der Pneumokokken und über die Theorie der Agglutination. Z Hyg Infektionskr 1:54–72
     [Google Scholar]
  17. O'Brien K. L., Nohynek H. 2003; Report from a WHO Working Group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae . Pediatr Infect Dis J 22:e1–e11
     [Google Scholar]
  18. O'Halloran D. M., Cafferkey M. T. 2005; Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine. J Clin Microbiol 43:3487–3490 [CrossRef]
     [Google Scholar]
  19. Pai R., Gertz R. E., Beall B. 2006; Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol 44:124–131 [CrossRef]
     [Google Scholar]
  20. Rivera-Olivero I. A., Bogaert D., Bello T., del Nogal B., Sluijter M., Hermans P. W., de Waard J. H. 2007; Pneumococcal carriage among indigenous Warao children in Venezuela: serotypes, susceptibility patterns, and molecular epidemiology. Clin Infect Dis 45:1427–1434 [CrossRef]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.006726-0
Loading
Multiplex PCR reveals a high rate of nasopharyngeal pneumococcal 7-valent conjugate vaccine serotypes co-colonizing indigenous Warao children in Venezuela
J. Med. Microbiol. 58, 584 (2009); https://doi.org/10.1099/jmm.0.006726-0
/content/journal/jmm/10.1099/jmm.0.006726-0
/content/journal/jmm/10.1099/jmm.0.006726-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error