1887

Abstract

Molecular detection and surveillance of the resistance genes harboured by are becoming increasingly important in assessing and controlling spread and colonization in hospitals, and in guiding the treatment of infections. This study analysed the resistance mechanisms of carbapenem-resistant clinical isolates of and identified the associated integron-borne metallo--lactamase (MBL)-encoding genes. Twenty-seven imipenem (IPM)-resistant clinical isolates of were divided into three groups according to their resistance levels to carbapenems. Strains bearing showed extremely high-level resistance to IPM, with MICs of 512–2048 μg ml. By comparison, strains bearing , and showed an intermediate level of resistance, with MICs of 32–256 μg ml. The non-MBL-producing strains showed a low level of resistance, with MICs of 8–32 μg ml. The same trend in resistance levels was also observed when resistance to other carbapenems, such as meropenem and panipenem, was determined. DNA sequencing showed that the MBL-encoding gene cassettes were carried by class 1 integrons. The , and gene cassettes were preceded by a hybrid promoter, TGGACA-N-TAAACT, and the gene cassette was preceded by a weak promoter, TGGACA-N-TAAGCT. Most of the MBL-encoding genes were linked to one or two resistance genes encoding aminoglycoside-modifying enzymes, such as , , , , , and , highlighting the multidrug-resistant properties of these clinical isolates.

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2009-08-01
2024-03-29
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