1887

Abstract

Several recent reports have suggested that community-associated meticillin-resistant (MRSA) clones, particularly those harbouring genes for Panton–Valentine leukocidin (PVL) or toxic shock syndrome toxin 1 (TSST-1), are increasingly responsible for infections in hospitals. Here, a retrospective study was carried out to investigate whether antimicrobial resistance patterns could be used to detect these pathogens in a French university hospital. Isolates were characterized by antimicrobial susceptibility testing, PCR profiling (PVL genes and ), PFGE typing and multilocus sequence typing. Demographic and clinical data were collected from all patients. For PVL-positive MRSA, the typical antimicrobial resistance pattern (susceptible to fluoroquinolones, non-susceptible to fusidic acid, kanamycin resistant and susceptible to gentamicin and tobramycin) had a sensitivity of 77.8 % and a positive predictive value (PPV) of 100 %. For -positive MRSA, the antimicrobial resistance pattern (susceptible to fluoroquinolones and non-susceptible to fusidic acid) had a sensitivity of 100 % and a PPV of 72.4 %. These results suggest that phenotypic rules based on antimicrobial resistance patterns are potentially useful for the detection of PVL- and -positive MRSA isolates.

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2009-10-01
2024-03-28
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References

  1. Bekkhoucha S. N., Cady A., Gautier P., Itim F., Donnio P. Y. 2009; A portrait of Staphylococcus aureus from the other side of the Mediterranean Sea: molecular characteristics of isolates from Western Algeria. Eur J Clin Microbiol Infect Dis 28:553–555 [CrossRef]
    [Google Scholar]
  2. Bertrand X., Thouverez M., Talon D. 2000; Antibiotic susceptibility and genotypic characterization of methicillin-resistant Staphylococcus aureus strains in eastern France. J Hosp Infect 46:280–287 [CrossRef]
    [Google Scholar]
  3. Bertrand X., Claude B., Chantelat P., Paolini M. C., Petitboulanger N., Julienne C., Bruand L., Huguenin Y., Mermet F., Talon D. 2004; Regional surveillance of the evolution of methicillin resistant Staphylococcus aureus (MRSA): what results for what teaching?. Med Mal Infect 34:264–269 (in French [CrossRef]
    [Google Scholar]
  4. Buck J. M., Como-Sabetti K., Harriman K. H., Danila R. N., Boxrud D. J., Glennen A., Lynfield R. 2005; Community-associated methicillin-resistant Staphylococcus aureus , Minnesota, 2000–2003. Emerg Infect Dis 11:1532–1538 [CrossRef]
    [Google Scholar]
  5. Chini V., Petinaki E., Foka A., Paratiras S., Dimitracopoulos G., Spiliopoulou I. 2006; Spread of Staphylococcus aureus clinical isolates carrying Panton–Valentine leukocidin genes during a 3-year period in Greece. Clin Microbiol Infect 12:29–34 [CrossRef]
    [Google Scholar]
  6. Cosgrove S. E., Qi Y., Kaye K. S., Harbarth S., Karchmer A. W., Carmeli Y. 2005; The impact of methicillin resistance in Staphylococcus aureus bacteremia on patient outcomes: mortality, length of stay, and hospital charges. Infect Control Hosp Epidemiol 26:166–174 [CrossRef]
    [Google Scholar]
  7. Dauwalder O., Lina G., Durand G., Bes M., Meugnier H., Jarlier V., Coignard B., Vandenesch F., Etienne J., Laurent F. 2008; Epidemiology of invasive methicillin-resistant Staphylococcus aureus clones collected in France in 2006 and 2007. J Clin Microbiol 46:3454–3458 [CrossRef]
    [Google Scholar]
  8. David M. Z., Glikman D., Crawford S. E., Peng J., King K. J., Hostetler M. A., Boyle-Vavra S., Daum R. S. 2008; What is community-associated methicillin-resistant Staphylococcus aureus ?. J Infect Dis 197:1235–1243 [CrossRef]
    [Google Scholar]
  9. Diep B. A., Carleton H. A., Chang R. F., Sensabaugh G. F., Perdreau-Remington F. 2006; Roles of 34 virulence genes in the evolution of hospital- and community-associated strains of methicillin-resistant Staphylococcus aureus . J Infect Dis 193:1495–1503 [CrossRef]
    [Google Scholar]
  10. Durand G., Bes M., Meugnier H., Enright M. C., Forey F., Liassine N., Wenger A., Kikuchi K., Lina G. other authors 2006; Detection of new methicillin-resistant Staphylococcus aureus clones containing the toxic shock syndrome toxin 1 gene responsible for hospital- and community-acquired infections in France. J Clin Microbiol 44:847–853 [CrossRef]
    [Google Scholar]
  11. Enright M. C., Day N. P., Davies C. E., Peacock S. J., Spratt B. G. 2000; Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus . J Clin Microbiol 38:1008–1015
    [Google Scholar]
  12. Jappe U., Heuck D., Strommenger B., Wendt C., Werner G., Altmann D., Witte W. 2008; Staphylococcus aureus in dermatology outpatients with special emphasis on community-associated methicillin-resistant strains. J Invest Dermatol 128:2655–2664 [CrossRef]
    [Google Scholar]
  13. Jarraud S., Mougel C., Thioulouse J., Lina G., Meugnier H., Forey F., Nesme X., Etienne J., Vandenesch F. 2002; Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease. Infect Immun 70:631–641 [CrossRef]
    [Google Scholar]
  14. Millar B. C., Loughrey A., Elborn J. S., Moore J. E. 2007; Proposed definitions of community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA). J Hosp Infect 67:109–113 [CrossRef]
    [Google Scholar]
  15. Otter J. A., French G. L. 2008; The emergence of community-associated methicillin-resistant Staphylococcus aureus at a London teaching hospital, 2000–2006. Clin Microbiol Infect 14:670–676 [CrossRef]
    [Google Scholar]
  16. Robert J., Etienne J., Bertrand X. 2005; Methicillin-resistant Staphylococcus aureus producing Panton–Valentine leukocidin in a retrospective case series from 12 French hospital laboratories, 2000–2003. Clin Microbiol Infect 11:585–587 [CrossRef]
    [Google Scholar]
  17. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239
    [Google Scholar]
  18. Thouverez M., Muller A., Hocquet D., Talon D., Bertrand X. 2003; Relationship between molecular epidemiology and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) in a French teaching hospital. J Med Microbiol 52:801–806 [CrossRef]
    [Google Scholar]
  19. Vandenesch F., Naimi T., Enright M. C., Lina G., Nimmo G. R., Heffernan H., Liassine N., Bes M., Greenland T. other authors 2003; Community-acquired methicillin-resistant Staphylococcus aureus carrying Panton–Valentine leukocidin genes: worldwide emergence. Emerg Infect Dis 9:978–984 [CrossRef]
    [Google Scholar]
  20. van der Mee-Marquet N., Lina G., Quentin R., Yaouanc-Lapalle H., Fievre C., Takahashi N., Etienne J. 2003; Staphylococcal exanthematous disease in a newborn due to a virulent methicillin-resistant Staphylococcus aureus strain containing the TSST-1 gene in Europe: an alert for neonatologists. J Clin Microbiol 41:4883–4884 [CrossRef]
    [Google Scholar]
  21. Witte W., Strommenger B., Cuny C., Heuck D., Nuebel U. 2007; Methicillin-resistant Staphylococcus aureus containing the Panton–Valentine leucocidin gene in Germany in 2005 and 2006. J Antimicrob Chemother 60:1258–1263 [CrossRef]
    [Google Scholar]
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