1887

Abstract

Recent investigations have suggested that CagA, a virulence factor of and known to have multiple genotypes, plays a critical role in the development of stomach cancer. However, the prevalence of positive strains and the genotypes have not been well studied in healthy individuals because of the difficulty in collecting gastric specimens. In the present study, we assessed the prevalence of infection with , particularly the strains with the East Asian genotype (which is more potent in causing gastric diseases), among healthy asymptomatic Japanese individuals by a noninvasive method using stool specimens. The antigen was detected in 40.3 % of healthy asymptomatic adult individuals (=186) enrolled in the study. For the detection and genotyping of the gene, DNA was extracted from the stool specimens of these individuals and analysed by PCR. We detected the East Asian genotype in the DNA samples of a significantly high number (63.1 %) of healthy asymptomatic Japanese individuals. These results indicate that a significant number of asymptomatic healthy Japanese individuals were infected with highly virulent .

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2009-09-01
2024-03-28
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References

  1. Asaka M., Kimura T., Kudo M., Takeda H., Mitani S., Miyazaki T., Miki K., Graham D. Y. 1992; Relationship of Helicobacter pylori to serum pepsinogens in an asymptomatic Japanese population. Gastroenterology 102:760–766
    [Google Scholar]
  2. Azuma T. 2004; Helicobacter pylori CagA protein variation associated with gastric cancer in Asia. J Gastroenterol 39:97–103 [CrossRef]
    [Google Scholar]
  3. Cardenas V. M., Dominguez D. C., Puentes F. A., Aragaki C. C., Goodman K. J., Graham D. Y., Fukuda Y. 2008; Evaluation of a novel stool native catalase antigen test for Helicobacter pylori infection in asymptomatic North American children. J Pediatr Gastroenterol Nutr 46:399–402 [CrossRef]
    [Google Scholar]
  4. Chattopadhyay S., Datta S., Chowdhury A., Chowdhury S., Mukhopadhyay A. K., Rajendran K., Bhattacharya S. K., Berg D. E., Nair G. B. 2002; Virulence genes in Helicobacter pylori strains from West Bengal residents with overt H. pylori -associated disease and healthy volunteers. J Clin Microbiol 40:2622–2625 [CrossRef]
    [Google Scholar]
  5. Covacci A., Censini S., Bugnoli M., Petracca R., Burroni D., Macchia G., Massone A., Papini E., Xiang Z. other authors 1993; Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci U S A 90:5791–5795 [CrossRef]
    [Google Scholar]
  6. Devi S. M., Ahmed I., Khan A. A., Rahman S. A., Alvi A., Sechi L. A., Ahmed N. 2006; Genomes of Helicobacter pylori from native Peruvians suggest admixture of ancestral and modern lineages and reveal a western type cag -pathogenicity island. BMC Genomics 7:191 [CrossRef]
    [Google Scholar]
  7. Fujisawa T., Kumagai T., Akamatsu T., Kiyosawa K., Matsunaga Y. 1999; Changes in seroepidemiological pattern of Helicobacter pylori and hepatitis A virus over the last 20 years in Japan. Am J Gastroenterol 94:2094–2099 [CrossRef]
    [Google Scholar]
  8. Fukase K., Kato M., Kikuchi S., Inoue K., Uemura N., Okamoto S., Terao S., Amagai K., Hayashi S., Asaka M. 2008; Effect of eradication of Helicobacter pylori on incidence of metachronous gastric carcinoma after endoscopic resection of early gastric cancer: an open-label, randomised controlled trial. Lancet 372:392–397 [CrossRef]
    [Google Scholar]
  9. Higashi H., Tsutsumi R., Fujita A., Yamazaki S., Asaka M., Azuma T., Hatakeyama M. 2002a; Biological activity of the Helicobacter pylori virulence factor CagA is determined by variation in the tyrosine phosphorylation sites. Proc Natl Acad Sci U S A 99:14428–14433 [CrossRef]
    [Google Scholar]
  10. Higashi H., Tsutsumi R., Muto S., Sugiyama T., Azuma T., Asaka M., Hatakeyama M. 2002b; SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein. Science 295:683–686 [CrossRef]
    [Google Scholar]
  11. Hirai I., Sasaki T., Fujimoto S., Moriyama T., Azuma T., Yamamoto Y. 2009; A method for assessment of Helicobacter pylori genotype using stool specimens. FEMS Immunol Med Microbiol 56:63–66 [CrossRef]
    [Google Scholar]
  12. Kanada R., Uchida T., Tsukamoto Y., Nguyen L. T., Hijiya N., Matsuura K., Kodama M., Okimoto T., Murakami K. other authors 2008; Genotyping of the cagA gene of Helicobacter pylori on immunohistochemistry with East Asian CagA-specific antibody. Pathol Int 58:218–225 [CrossRef]
    [Google Scholar]
  13. Molnar B., Szoke D., Ruzsovics A., Tulassay Z. 2008; Significantly elevated Helicobacter pylori density and different genotype distribution in erosions as compared with normal gastric biopsy specimen detected by quantitative real-time PCR. Eur J Gastroenterol Hepatol 20:305–313 [CrossRef]
    [Google Scholar]
  14. Naito M., Yamazaki T., Tsutsumi R., Higashi H., Onoe K., Yamazaki S., Azuma T., Hatakeyama M. 2006; Influence of EPIYA-repeat polymorphism on the phosphorylation-dependent biological activity of Helicobacter pylori CagA. Gastroenterology 130:1181–1190 [CrossRef]
    [Google Scholar]
  15. Suzuki N., Wakasugi M., Nakaya S., Okada K., Mochida R., Sato M., Kajiyama H., Takahashi R., Hirata H. other authors 2002; Production and application of new monoclonal antibodies specific for a fecal Helicobacter pylori antigen. Clin Diagn Lab Immunol 9:75–78
    [Google Scholar]
  16. Vilaichone R. K., Mahachai V., Tumwasorn S., Wu J. Y., Graham D. Y., Yamaoka Y. 2004; Molecular epidemiology and outcome of Helicobacter pylori infection in Thailand: a cultural cross roads. Helicobacter 9:453–459 [CrossRef]
    [Google Scholar]
  17. Yamagata H., Kiyohara Y., Aoyagi K., Kato I., Iwamoto H., Nakayama K., Shimizu H., Tanizaki Y., Arima H. other authors 2000; Impact of Helicobacter pylori infection on gastric cancer incidence in a general Japanese population: the Hisayama study. Arch Intern Med 160:1962–1968 [CrossRef]
    [Google Scholar]
  18. Yamaoka Y., Malaty H. M., Osato M. S., Graham D. Y. 2000a; Conservation of Helicobacter pylori genotypes in different ethnic groups in Houston, Texas. J Infect Dis 181:2083–2086 [CrossRef]
    [Google Scholar]
  19. Yamaoka Y., Osato M. S., Sepulveda A. R., Gutierrez O., Figura N., Kim J. G., Kodama T., Kashima K., Graham D. Y. 2000b; Molecular epidemiology of Helicobacter pylori : separation of H. pylori from East Asian and non-Asian countries. Epidemiol Infect 124:91–96 [CrossRef]
    [Google Scholar]
  20. Yamaoka Y., Kato M., Asaka M. 2008; Geographic differences in gastric cancer incidence can be explained by differences between Helicobacter pylori strains. Intern Med 47:1077–1083 [CrossRef]
    [Google Scholar]
  21. Yamazaki S., Kato S., Matsukura N., Ohtani M., Ito Y., Suto H., Yamazaki Y., Yamakawa A., Tokudome S. other authors 2005a; Identification of Helicobacter pylori and the cagA genotype in gastric biopsies using highly sensitive real-time PCR as a new diagnostic tool. FEMS Immunol Med Microbiol 44:261–268 [CrossRef]
    [Google Scholar]
  22. Yamazaki S., Yamakawa A., Okuda T., Ohtani M., Suto H., Ito Y., Yamazaki Y., Keida Y., Higashi H. other authors 2005b; Distinct diversity of vacA , cagA , and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan. J Clin Microbiol 43:3906–3916 [CrossRef]
    [Google Scholar]
  23. Yoshida S., Kozu T., Gotoda T., Saito D. 2006; Detection and treatment of early cancer in high-risk populations. Best Pract Res Clin Gastroenterol 20:745–765 [CrossRef]
    [Google Scholar]
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