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Volume 59, Issue 2

O1 Ogawa detoxified lipopolysaccharide structures as inducers of cytokines and oxidative species in macrophages Free

Abstract

Multidrug resistance in several strains of has encouraged anti-cholera vaccine developmental attempts using various subcellular moieties. In order to examine the immunological efficacy of detoxified LPS (dLPS)-derived saccharide immunogens, activation of mouse peritoneal macrophages (MΦs) was investigated. The immunomodulatory effect was evaluated via induction of the pro-inflammatory cytokines tumour necrosis factor-, interleukin (IL)-1 and IL-6 and acceleration of nitric oxide (NO) and reactive oxygen species (ROS). Immunologically active structures triggered mouse peritoneal MΦs to secrete cytokines and release NO/ROS, even at concentrations as low as 12.5 μg ml. It was found that the O-specific polysaccharide moiety was more immunologically efficient than the glycolipid one, probably due to the position of 3-deoxy--manno-octulosonic acid. The results revealed effective structure–immunomodulating relationships of dLPS-derived moieties that are desirable in subcellular anti-cholera vaccine design.

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Keyword(s): DeOAc-LPS, de-O-acetylated glycolipid , dLPS, detoxified LPS , EU, endotoxin unit , GM-CSF, granulocyte–macrophage colony stimulating factor , IFN, interferon , IL, interleukin , KDO, 3-deoxy-d-manno-octulosonic acid , MΦ, macrophage , O-SP, O-specific polysaccharide , ROS, reactive oxygen species and TNF-α, tumour necrosis factor-α
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2010-02-01
2024-04-19
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Vibrio cholerae O1 Ogawa detoxified lipopolysaccharide structures as inducers of cytokines and oxidative species in macrophages
J. Med. Microbiol. 59, 158 (2010); https://doi.org/10.1099/jmm.0.013599-0
/content/journal/jmm/10.1099/jmm.0.013599-0
/content/journal/jmm/10.1099/jmm.0.013599-0
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