%0 Journal Article %A Zhang, Jian %A Abadia, Edgar %A Refregier, Guislaine %A Tafaj, Silva %A Boschiroli, Maria Laura %A Guillard, Bertrand %A Andremont, Antoine %A Ruimy, Raymond %A Sola, Christophe %T Mycobacterium tuberculosis complex CRISPR genotyping: improving efficiency, throughput and discriminative power of ‘spoligotyping’ with new spacers and a microbead-based hybridization assay %D 2010 %J Journal of Medical Microbiology, %V 59 %N 3 %P 285-294 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.016949-0 %K VNTR, variable number of tandem repeats %K EDC, N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide %K TMAC, tetra-methyl ammonium chloride %K HGDI, Hunter and Gaston index; IGEPE, infection, genetics, emerging pathogens, evolution %K CAS, Central Asian %K SNP, single nucleotide polymorphism %K DVR, direct variable repeat %K DR, direct repeat %K LAM, Latin-American and Mediterranean %K S : , N, signal to noise ratio %K MDR, multidrug resistant %K CRISPR, clustered regularly interspaced palindromic repeats %K EAI, East African–Indian %K MTC, Mycobacterium tuberculosis complex %K PGG, principal genetic group %I Microbiology Society, %X The aims of the present study were to implement a microbead-based ‘spoligotyping’ technique and to evaluate improvements by the addition of a panel of 25 extra spacers that we expected to provide an increased resolution on principal genetic group 1 (PGG 1) strains. We confirmed the high sensitivity and reproducibility of the classical technique using the 43 spacer panel and we obtained perfect agreement between the membrane-based and the microbead-based techniques. We further demonstrated an increase in the discriminative power of an extended 68 spacer format for differentiation of PGG 1 clinical isolates, in particular for the East African–Indian clade. Finally, we define a limited yet highly informative reduced 10 spacer panel set which could offer a more cost-effective option for implementation in resource-limited countries and that could decrease the need for additional VNTR (variable number of tandem repeats) genotyping work in molecular epidemiological studies. We also present an economic analysis comparing membrane-based and microbead-based techniques. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.016949-0