@article{mbs:/content/journal/jmm/10.1099/jmm.0.017624-0, author = "Morita, Masatomo and Ohnishi, Makoto and Arakawa, Eiji and Yamamoto, Shouji and Nair, G. Balakrish and Matsushita, Shigeru and Yokoyama, Keiko and Kai, Akemi and Seto, Kazuko and Watanabe, Haruo and Izumiya, Hidemasa", title = "Emergence and genetic diversity of El Tor Vibrio cholerae O1 that possess classical biotype ctxB among travel-associated cases of cholera in Japan", journal= "Journal of Medical Microbiology", year = "2010", volume = "59", number = "6", pages = "708-712", doi = "https://doi.org/10.1099/jmm.0.017624-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.017624-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "MLVA, multilocus variable-number tandem repeat analysis", keywords = "MAMA-PCR, mismatch amplification mutation assay PCR", abstract = " Vibrio cholerae O1 are classified into two biotypes, classical and El Tor, each encoding a biotype-specific cholera toxin. However, El Tor strains have recently emerged with a classical cholera-toxin genotype (El Tor variant). We characterized El Tor strains of V. cholerae O1 from travel-associated cases of cholera in Japan isolated from 1991 to 2006 by cholera toxin B subunit gene (ctxB) typing and by molecular epidemiological methods. ctxB in the biotype El Tor shifted from the El Tor-specific type to the classical-specific type around 1993, and this type fully dominated the later half of the 1990s. Based on the results of PFGE and multilocus variable-number tandem repeat analysis, strains of the classical biotype remained diverse from those of El Tor biotype. The El Tor biotype strains formed multiple minor clusters and intermingled with each other irrespective of their origins and toxin types. El Tor variant strains are widespread in Asian countries and show significant genetic diversity, indicating that their spread is a result of multiclonal expansion rather than spread from a single clone.", }