Bactericidal effects of non-thermal argon plasma in vitro, in biofilms and in the animal model of infected wounds Ermolaeva, Svetlana A. and Varfolomeev, Alexander F. and Chernukha, Marina Yu. and Yurov, Dmitry S. and Vasiliev, Mikhail M. and Kaminskaya, Anastasya A. and Moisenovich, Mikhail M. and Romanova, Julia M. and Murashev, Arcady N. and Selezneva, Irina I. and Shimizu, Tetsuji and Sysolyatina, Elena V. and Shaginyan, Igor A. and Petrov, Oleg F. and Mayevsky, Evgeny I. and Fortov, Vladimir E. and Morfill, Gregor E. and Naroditsky, Boris S. and Gintsburg, Alexander L.,, 60, 75-83 (2011), doi = https://doi.org/10.1099/jmm.0.020263-0, publicationName = Microbiology Society, issn = 0022-2615, abstract= Non-thermal (low-temperature) physical plasma is under intensive study as an alternative approach to control superficial wound and skin infections when the effectiveness of chemical agents is weak due to natural pathogen or biofilm resistance. The purpose of this study was to test the individual susceptibility of pathogenic bacteria to non-thermal argon plasma and to measure the effectiveness of plasma treatments against bacteria in biofilms and on wound surfaces. Overall, Gram-negative bacteria were more susceptible to plasma treatment than Gram-positive bacteria. For the Gram-negative bacteria Pseudomonas aeruginosa, Burkholderia cenocepacia and Escherichia coli, there were no survivors among the initial 105 c.f.u. after a 5 min plasma treatment. The susceptibility of Gram-positive bacteria was species- and strain-specific. Streptococcus pyogenes was the most resistant with 17 % survival of the initial 105 c.f.u. after a 5 min plasma treatment. Staphylococcus aureus had a strain-dependent resistance with 0 and 10 % survival from 105 c.f.u. of the Sa 78 and ATCC 6538 strains, respectively. Staphylococcus epidermidis and Enterococcus faecium had medium resistance. Non-ionized argon gas was not bactericidal. Biofilms partly protected bacteria, with the efficiency of protection dependent on biofilm thickness. Bacteria in deeper biofilm layers survived better after the plasma treatment. A rat model of a superficial slash wound infected with P. aeruginosa and the plasma-sensitive Staphylococcus aureus strain Sa 78 was used to assess the efficiency of argon plasma treatment. A 10 min treatment significantly reduced bacterial loads on the wound surface. A 5-day course of daily plasma treatments eliminated P. aeruginosa from the plasma-treated animals 2 days earlier than from the control ones. A statistically significant increase in the rate of wound closure was observed in plasma-treated animals after the third day of the course. Wound healing in plasma-treated animals slowed down after the course had been completed. Overall, the results show considerable potential for non-thermal argon plasma in eliminating pathogenic bacteria from biofilms and wound surfaces., language=, type=