1887

Abstract

Oxoid MRSA Agar and bioMérieux MRSA ID medium were evaluated for their ability to identify meticillin-resistant (MRSA) in clinical samples. Nasal and throat samples ( = 629) were taken from veterinarians and their household members. The sensitivities of MRSA Agar and MRSA ID medium after 20 h of incubation were 63.6 and 64.5 %, and the specificities were 94.1 and 99.4 %, respectively. After an enrichment step, the sensitivities increased to 96.3 and 97.2 %, but the specificities decreased to 88.7 and 98.5 %, respectively. MRSA Agar and MRSA ID medium are both sensitive methods for the screening of MRSA in combination with broth enrichment, but positive results require confirmation.

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2011-07-01
2024-04-28
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References

  1. Archer G. L. 1998; Staphylococcus aureus: a well-armed pathogen. Clin Infect Dis 26:1179–1181 [View Article][PubMed]
    [Google Scholar]
  2. Brown D. F. J., Edwards D. I., Hawkey P. M., Morrison D., Ridgway G. L., Towner K. J., Wren M. W. D. on behalf of the Joint Working Party of the British Society for Antimicrobial Chemotherapy, Hospital Infection Society and Infection Control Nurses Association 2005; Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother 56:1000–1018 [View Article][PubMed]
    [Google Scholar]
  3. Kluytmans J. A., Van Griethuysen A., Willemse P., Van Keulen P. 2002; Performance of CHROMagar selective medium and oxacillin resistance screening agar base for identifying Staphylococcus aureus and detecting methicillin resistance. J Clin Microbiol 40:2480–2482 [View Article][PubMed]
    [Google Scholar]
  4. Kluytmans-Vandenbergh M. F., Kluytmans J. A. 2006; Community-acquired methicillin-resistant Staphylococcus aureus: current perspectives. Clin Microbiol Infect 12:Suppl. 19–15 [View Article][PubMed]
    [Google Scholar]
  5. Malhotra-Kumar S., Abrahantes J. C., Sabiiti W., Lammens C., Vercauteren G., Ieven M., Molenberghs G., Aerts M., Goossens H. MOSAR WP2 Study Team 2010; Evaluation of chromogenic media for detection of methicillin-resistant Staphylococcus aureus . J Clin Microbiol 48:1040–1046 [View Article][PubMed]
    [Google Scholar]
  6. Merlino J., Leroi M., Bradbury R., Veal D., Harbour C. 2000; New chromogenic identification and detection of Staphylococcus aureus and methicillin-resistant S. aureus . J Clin Microbiol 38:2378–2380[PubMed]
    [Google Scholar]
  7. Muto C. A., Jernigan J. A., Ostrowsky B. E., Richet H. M., Jarvis W. R., Boyce J. M., Farr B. M. 2003; SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and Enterococcus . Infect Control Hosp Epidemiol 24:362–386 [View Article][PubMed]
    [Google Scholar]
  8. NCCLS 1997 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, 4th edn. Approved Standard M7–A4 Wayne, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  9. Nonhoff C., Denis O., Brenner A., Buidin P., Legros N., Thiroux C., Dramaix M., Struelens M. J. 2009; Comparison of three chromogenic media and enrichment broth media for the detection of methicillin-resistant Staphylococcus aureus from mucocutaneous screening specimens. Eur J Clin Microbiol Infect Dis 28:363–369 [View Article][PubMed]
    [Google Scholar]
  10. Noskin G. A., Rubin R. J., Schentag J. J., Kluytmans J., Hedblom E. C., Jacobson C., Smulders M., Gemmen E., Bharmal M. 2007; National trends in Staphylococcus aureus infection rates: impact on economic burden and mortality over a 6-year period (1998-2003). Clin Infect Dis 45:1132–1140 [View Article][PubMed]
    [Google Scholar]
  11. Peterson J. F., Riebe K. M., Hall G. S., Wilson D., Whittier S., Palavecino E., Ledeboer N. A. 2010; Spectra MRSA, a new chromogenic agar medium to screen for methicillin-resistant Staphylococcus aureus . J Clin Microbiol 48:215–219 [View Article][PubMed]
    [Google Scholar]
  12. van Griethuysen A. J., Pouw M., van Leeuwen N., Heck M., Willemse P., Buiting A., Kluytmans J. 1999; Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus . J Clin Microbiol 37:2789–2792[PubMed]
    [Google Scholar]
  13. van Loo I., Huijsdens X., Tiemersma E., de Neeling A., van de Sande-Bruinsma N., Beaujean D., Voss A., Kluytmans J. 2007; Emergence of methicillin-resistant Staphylococcus aureus of animal origin in humans. Emerg Infect Dis 13:1834–1839[PubMed] [CrossRef]
    [Google Scholar]
  14. Voss A., Loeffen F., Bakker J., Klaassen C., Wulf M. 2005; Methicillin-resistant Staphylococcus aureus in pig farming. Emerg Infect Dis 11:1965–1966[PubMed] [CrossRef]
    [Google Scholar]
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