1887

Abstract

Strongyloidiasis is a neglected disease that is prevalent mainly in tropical and subtropical regions. It is caused by intestinal nematodes of the genus . Due to the rise in worldwide travel, infections are increasingly encountered in non-endemic regions. Diagnosis is hampered by insensitive and laborious detection methods. A universal species real-time PCR was developed with an internal competitive control system. The 95 % limit of detection as determined by probit analysis was one larva per PCR equivalent to 100 larvae per 200 mg stool. The assay proved to be 100 % specific as assessed using a panel of parasites and bacteria and thus might be useful in the diagnostic setting as well as for research.

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2011-04-01
2024-03-29
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References

  1. Bethony J., Brooker S., Albonico M., Geiger S. M., Loukas A., Diemert D., Hotez P. J. 2006; Soil-transmitted helminth infections: ascariasis, trichuriasis, and hookworm. Lancet 367:1521–1532 [CrossRef]
    [Google Scholar]
  2. Blessmann J., Buss H., Nu P. A., Dinh B. T., Ngo Q. T., Van A. L., Alla M. D., Jackson T. F., Ravdin J. I., Tannich E. 2002; Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples. J Clin Microbiol 40:4413–4417 [CrossRef]
    [Google Scholar]
  3. García L. S., Bruckner D. A. 2001 Diagnostic Medical Parasitology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Igual-Adell R., Oltra-Alcaraz C., Soler-Company E., Sánchez-Sánchez P., Matogo-Oyana J., Rodríguez-Calabuig D. 2004; Efficacy and safety of ivermectin and thiabendazole in the treatment of strongyloidiasis. Expert Opin Pharmacother 5:2615–2619 [CrossRef]
    [Google Scholar]
  5. Marcos L. A., Terashima A., Dupont H. L., Gotuzzo E. 2008; Strongyloides hyperinfection syndrome: an emerging global infectious disease. Trans R Soc Trop Med Hyg 102:314–318 [CrossRef]
    [Google Scholar]
  6. Nuesch R., Zimmerli L., Stockli R., Gyr N., Hatz C. F. R. 2005; Imported strongyloidosis: a longitudinal analysis of 31 cases. J Travel Med 12:80–84
    [Google Scholar]
  7. Olsen A., van Lieshout L., Marti H., Polderman T., Polman K., Steinmann P., Stothard R., Thybo S., Verweij J. J., Magnussen P. 2009; Strongyloidiasis – the most neglected of the neglected tropical diseases?. Trans R Soc Trop Med Hyg 103:967–972 [CrossRef]
    [Google Scholar]
  8. Panning M., Kramme S., Petersen N., Drosten C. 2007; High throughput screening for spores and vegetative forms of pathogenic B. anthracis by an internally controlled real-time PCR assay with automated DNA preparation. Med Microbiol Immunol (Berl) 196:41–50 [CrossRef]
    [Google Scholar]
  9. Panning M., Kilwinski J., Greiner-Fischer S., Peters M., Kramme S., Frangoulidis D., Meyer H., Henning K., Drosten C. 2008; High throughput detection of Coxiella burnetii by real-time PCR with internal control system and automated DNA preparation. BMC Microbiol 8:77 [CrossRef]
    [Google Scholar]
  10. Ravi V., Ramachandran S., Thompson R. W., Andersen J. F., Neva F. A. 2002; Characterization of a recombinant immunodiagnostic antigen (NIE) from Strongyloides stercoralis L3-stage larvae. Mol Biochem Parasitol 125:73–81 [CrossRef]
    [Google Scholar]
  11. Rodrigues R. M., de Oliveira M. C., Sopelete M. C., Silva D. A., Campos D. M., Taketomi E. A., Costa-Cruz J. M. 2007; IgG1, IgG4, and IgE antibody responses in human strongyloidiasis by ELISA using Strongyloides ratti saline extract as heterologous antigen. Parasitol Res 101:1209–1214 [CrossRef]
    [Google Scholar]
  12. Siddiqui A. A., Berk S. L. 2001; Diagnosis of Strongyloides stercoralis infection. Clin Infect Dis 33:1040–1047 [CrossRef]
    [Google Scholar]
  13. Silva L. P., Barcelos I. S., Passos-Lima A. B., Espindola F. S., Campos D. M., Costa-Cruz J. M. 2003; Western blotting using Strongyloides ratti antigen for the detection of IgG antibodies as confirmatory test in human strongyloidiasis. Mem Inst Oswaldo Cruz 98:687–691 [CrossRef]
    [Google Scholar]
  14. Sudarshi S., Stümpfle R., Armstrong M., Ellman T., Parton S., Krishnan P., Chiodini P. L., Whitty C. J. 2003; Clinical presentation and diagnostic sensitivity of laboratory tests for Strongyloides stercoralis in travellers compared with immigrants in a non-endemic country. Trop Med Int Health 8:728–732 [CrossRef]
    [Google Scholar]
  15. ten Hove R. J., van Esbroeck M., Vervoort T., van den Ende J., van Lieshout L., Verweij J. J. 2009; Molecular diagnostics of intestinal parasites in returning travellers. Eur J Clin Microbiol Infect Dis 28:1045–1053 [CrossRef]
    [Google Scholar]
  16. Verweij J. J., van Lieshout L., Blotkamp C., Brienen E. A., van Duivenvoorden S., van Esbroeck M., Polderman A. M. 2000; Differentiation of Entamoeba histolytica and Entamoeba dispar using PCR-SHELA and comparison of antibody response. Arch Med Res 31:S44–S46 [CrossRef]
    [Google Scholar]
  17. Verweij J. J., Canales M., Polman K., Ziem J., Brienen E. A., Polderman A. M., van Lieshout L. 2009; Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR. Trans R Soc Trop Med Hyg 103:342–346 [CrossRef]
    [Google Scholar]
  18. Viney M. E. 1999; Exploiting the life cycle of Strongyloides ratti . Parasitol Today 15:231–235 [CrossRef]
    [Google Scholar]
  19. Viney M. E., Lok J. B. 2007; Strongyloides spp. In WormBook. Edited by The C. elegans Research Community [View Article] http://www.wormbook.org
    [Google Scholar]
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