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Volume 60, Issue 3

Simultaneous carriage of multiple genotypes of in children Free

Abstract

The co-existence of multiple genotypes in colonization by has not been fully investigated. The aim of this study was to evaluate the heterogeneity of carriage in children. We evaluated 125 nasal and perianal swab samples that were positive for from 76 children scheduled for elective surgery. For each sample, at least four colonies with the same or different morphotypes were selected for analysis. Multiple-locus variable-number tandem-repeat fingerprinting was used to determine the genetic relatedness and to characterize the clonality of the strains. Of the 125 swabs, 91 (73 %) contained meticillin-sensitive (MSSA), 8 (6 %) contained meticillin-resistant (MRSA), and 26 (21 %) contained MSSA and MRSA simultaneously. A total of 738 strains were evaluated with a mean of 6 colonies (range 4–15) picked from each culture. Of the 125 swabs, 32 (26 %) samples contained two genetically distinct strains and 6 (5 %) contained three different genotypes. Multiple strains simultaneously carried by individual children were genetically unrelated to each other. We concluded that the co-existence of multiple genotypes of was common. The significance of multiple carriage is yet to be determined, but this intraspecies interplay could be important to pathogenicity and virulence in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): MLVF, multiple-locus variable-number tandem-repeat fingerprinting , MRSA, meticillin-resistant Staphylococcus aureus and MSSA, meticillin-sensitive Staphylococcus aureus
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/content/journal/jmm/10.1099/jmm.0.025841-0
2011-03-01
2024-04-19
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Simultaneous carriage of multiple genotypes of Staphylococcus aureus in children
J. Med. Microbiol. 60, 317 (2011); https://doi.org/10.1099/jmm.0.025841-0
/content/journal/jmm/10.1099/jmm.0.025841-0
/content/journal/jmm/10.1099/jmm.0.025841-0
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