RT Journal Article SR Electronic(1) A1 Abdel-Haq, Nahed A1 Amjad, Muhammad A1 McGrath, Eric A1 Chearskul, Pimpanada A1 Amer, Ahdi A1 Salimnia, Hossein A1 Asmar, Basim I.YR 2011 T1 Emergence of human rotavirus genotype G9 in metropolitan Detroit between 2007 and 2009 JF Journal of Medical Microbiology, VO 60 IS 6 SP 761 OP 767 DO https://doi.org/10.1099/jmm.0.026807-0 PB Microbiology Society, SN 1473-5644, AB Between January 2007 and April 2009, rotavirus (RV)-positive stool samples from 238 children with acute gastroenteritis, seen at Children’s Hospital of Michigan in Detroit, USA, were collected and RV genotyping was performed. G and P genotypes were determined by RT-PCR and nucleotide sequencing was conducted on selected G9 and P[6] strains. Correlation between the severity of gastroenteritis episode and the infecting G genotype was done using a 14-point scoring system. The predominant G genotype was G9 (39.5 %), followed by G1 (35.3 %) and G4 (15.5 %), while P[8] was the most prevalent P genotype (66.5 %), followed by P[4] (21.9 %) and P[6] (11.2 %). The gene combinations G1P[8] and G9P[8] were the most prevalent (21.4 % and 20.6 %, respectively), followed by G4P[8] (13 %) and G9P[6] (8.8 %). Immunization data showed that only 17/238 (7.1 %) children received ≥one dose of RV vaccine (the pentavalent vaccine RotaTeq or the monovalent vaccine Rotarix) and that 10/17 were infected with G4P[8] strains. Severity of RV gastroenteritis episodes was not related to the infecting G genotype. Our results suggest a high proportion of genotype G9 strains in combination with P[8], P[6] and P[4] specificity circulating in the metropolitan Detroit area. While the protective efficacy of the RV vaccines has been demonstrated against G9P[8] strains, the level of cross-protection offered by the vaccines against G9 strains with P[6] and P[4] genotypes in the Detroit paediatric population remains to be determined., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.026807-0