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Volume 60, Issue 7

The prevalence of urogenital micro-organisms detected by a multiplex PCR–reverse line blot assay in women attending three sexual health clinics in Sydney, Australia Free

Abstract

This study used a previously described multiplex PCR-based reverse line blot (mPCR/RLB) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely , , , , , , , , , , , herpes simplex virus types 1 and 2, and human adenovirus. First-voided urine specimens and endocervical and self-collected vaginal swabs from each of 216 women attending three sexual health clinics in Sydney, Australia, were tested and the results were compared with those of reference methods for each organism. One hundred and sixty-eight women (77.7 %) had at least one and 105 (48.6 %) had more than one target organism, most commonly and spp. The prevalence of each of the four known sexually transmissible pathogens was <5 %. Of the 216 women, 111 (51.4 %) reported at least one symptom consistent with genital or urethral infection, including discharge, pain or discomfort. Only was detected more frequently in women with symptoms ( = 0.05). The specificity of the mPCR/RLB assay compared with that of the reference methods for each organism and for all specimen types was 100 %. The mean sensitivities of the mPCR/RLB assay compared with those of the reference methods for self-collected vaginal swabs, cervical swabs and first-voided urine specimens for all organisms were 99.3, 98.1 and 84.6 %, respectively; however, these differences were not significant. There were no differences in sensitivities between specimen types for , , and , although all were found infrequently. Overall, the mPCR/RLB platform was found to be an accurate testing platform in a sexual health clinic setting.

  • Received:
  • Accepted:
  • Published Online:
© 2011 SGM
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2011-07-01
2024-04-23
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The prevalence of urogenital micro-organisms detected by a multiplex PCR–reverse line blot assay in women attending three sexual health clinics in Sydney, Australia
J. Med. Microbiol. 60, 1010 (2011); https://doi.org/10.1099/jmm.0.029108-0
/content/journal/jmm/10.1099/jmm.0.029108-0
/content/journal/jmm/10.1099/jmm.0.029108-0
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