RT Journal Article SR Electronic(1) A1 Rousseau, Clotilde A1 Lemée, Ludovic A1 Le Monnier, Alban A1 Poilane, Isabelle A1 Pons, Jean-Louis A1 Collignon, AnneYR 2011 T1 Prevalence and diversity of Clostridium difficile strains in infants JF Journal of Medical Microbiology, VO 60 IS 8 SP 1112 OP 1118 DO https://doi.org/10.1099/jmm.0.029736-0 PB Microbiology Society, SN 1473-5644, AB During early infancy asymptomatic intestinal colonization by Clostridium difficile is frequent. To update information on infant colonization prevalence and to characterize infant strains, in terms of their virulence factors and their phylogenetic diversity, a prospective screening of C. difficile in the stools of infants 0 to 2 years old was conducted at Jean Verdier Hospital (Hôpital Jean Verdier) over an 18 month period. C. difficile was screened by toxigenic culture, and molecular characterization was performed by PCR-ribotyping and multilocus sequence typing (MLST). The overall C. difficile colonization prevalence was 33.7 % (99/294). The colonization rate by a toxigenic strain was 7.1 % (21/294). Community-acquired C. difficile accounted for 66.7 % (66/99) of cases. Molecular typing was performed on 90 isolates from Jean Verdier Hospital and 8 additional isolates from another hospital in Versailles (Centre Hospitalier de Versailles). Among these isolates, 23 were toxigenic (21 tcdA+/tcdB + and 2 tcdA−/tcdB +). All the isolates were negative for the binary toxin genes. Seventeen PCR ribotypes (PRs) were identified, with five PRs accounting for 82.7 % (81/98) of the isolates. MLST generated 15 different sequence types (STs). The predominant genotype, PRJV11-ST38 (33.7 %), included only non-toxigenic strains. Toxigenic strains were distributed in eight genotypes. Neither PR027-ST3, nor PR078/126-ST49 were identified but some PRs/STs corresponded to well-known adult infectious strains. These results indicate that infants are widely colonized by non-toxigenic strains. However, toxigenic adult infectious strains circulate in asymptomatic infants even in the community; thus, infants may be a reservoir for adult infectious strains., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.029736-0