1887

Abstract

During a 24 month period from 2007 to 2009, 174 faecal specimens from horses in Australia (predominantly from Western Australia) were tested for . was isolated from 14 (23 %) of 62 diarrhoeal animals (including 10 foals) and from none of 112 healthy adult horses. These isolates were toxin profiled by PCR for toxin A, toxin B and binary toxin, and ribotyped. Ten of the equine isolates were ABCDT. Other toxin profiles detected were ABCDT (one isolate), ABCDT (two isolates) and ABCDT (three isolates). There were six different ribotypes detected in the horses, ribotype 012 being the most common with six isolates. Two horses (one adult and one foal) had two strains of isolated on different days. These strains had the same toxin profile but different ribotypes. None of the equine isolates was ribotype 078, which is ABCDT and a significant cause of animal disease overseas. All isolates were susceptible to metronidazole and vancomycin. These results suggest that the epidemiology of in horses in Australia is currently similar to that in other parts of the world, but requires further surveillance to monitor changes.

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2011-08-01
2024-03-28
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References

  1. Ackermann G., Tang-Feldman Y. J., Schaumann R., Henderson J. P., Rodloff A. C., Silva J., Cohen S. H. 2003; Antecedent use of fluoroquinolones is associated with resistance to moxifloxacin in Clostridium difficile . Clin Microbiol Infect 9:526–530 [View Article][PubMed]
    [Google Scholar]
  2. Arroyo L. G., Stämpfli H. R., Weese J. S. 2006; Potential role of Clostridium difficile as a cause of duodenitis-proximal jejunitis in horses. J Med Microbiol 55:605–608 [View Article][PubMed]
    [Google Scholar]
  3. Bauer M. P., Notermans D. W., Van Benthem B. H. B., Brazier J. S., Wilcox M. H., Rupnik M., Monnet D. L., Van Dissel J. T., Kuijper E. J. ECDIS Study Group 2011; Clostridium difficile infection in Europe: a hospital-based survey. Lancet 377:63–73 [View Article][PubMed]
    [Google Scholar]
  4. Båverud V. 2004; Clostridium difficile diarrhea: infection control in horses. Vet Clin North Am Equine Pract 20:615–630 [View Article][PubMed]
    [Google Scholar]
  5. Båverud V., Gustafsson A., Franklin A., Lindholm A., Gunnarsson A. 1997; Clostridium difficile associated with acute colitis in mature horses treated with antibiotics. Equine Vet J 29:279–284 [View Article][PubMed]
    [Google Scholar]
  6. Båverud V., Franklin A., Gunnarsson A., Gustafsson A., Hellander-Edman A. 1998; Clostridium difficile associated with acute colitis in mares when their foals are treated with erythromycin and rifampicin for Rhodococcus equi pneumonia. Equine Vet J 30:482–488 [View Article][PubMed]
    [Google Scholar]
  7. Båverud V., Gustafsson A., Franklin A., Aspán A., Gunnarsson A. 2003; Clostridium difficile: prevalence in horses and environment, and antimicrobial susceptibility. Equine Vet J 35:465–471 [View Article][PubMed]
    [Google Scholar]
  8. Bowman R. A., Riley T. V. 1988; Laboratory diagnosis of Clostridium difficile-associated diarrhoea. Eur J Clin Microbiol Infect Dis 7:476–484 [View Article][PubMed]
    [Google Scholar]
  9. Bowman R. A., Arrow S. A., Riley T. V. 1986; Latex particle agglutination for detecting and identifying Clostridium difficile . J Clin Pathol 39:212–214 [View Article][PubMed]
    [Google Scholar]
  10. Brazier J. S. 2001; Typing of Clostridium difficile . Clin Microbiol Infect 7:428–431 [View Article][PubMed]
    [Google Scholar]
  11. Carroll S. M., Bowman R. A., Riley T. V. 1983; A selective broth for Clostridium difficile . Pathology 15:165–167 [View Article][PubMed]
    [Google Scholar]
  12. Goorhuis A., Bakker D., Corver J., Debast S. B., Harmanus C., Notermans D. W., Bergwerff A. A., Dekker F. W., Kuijper E. J. 2008; Emergence of Clostridium difficile infection due to a new hypervirulent strain, polymerase chain reaction ribotype 078. Clin Infect Dis 47:1162–1170 [View Article][PubMed]
    [Google Scholar]
  13. Jones R. L., Adney W. S., Shideler R. K. 1987; Isolation of Clostridium difficile and detection of cytotoxin in the feces of diarrheic foals in the absence of antimicrobial treatment. J Clin Microbiol 25:1225–1227[PubMed]
    [Google Scholar]
  14. Keel K., Brazier J. S., Post K. W., Weese S., Songer J. G. 2007; Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J Clin Microbiol 45:1963–1964 [View Article][PubMed]
    [Google Scholar]
  15. Levett P. N. 1986; Clostridium difficile in habitats other than the human gastro-intestinal tract. J Infect 12:253–263 [View Article][PubMed]
    [Google Scholar]
  16. Loo V. G., Poirier L., Miller M. A., Oughton M., Libman M. D., Michaud S., Bourgault A. M., Nguyen T., Frenette C. et al. 2005; A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. N Engl J Med 353:2442–2449 [View Article][PubMed]
    [Google Scholar]
  17. Madewell B. R., Tang Y. J., Jang S., Madigan J. E., Hirsh D. C., Gumerlock P. H., Silva J. Jr 1995; Apparent outbreaks of Clostridium difficile-associated diarrhea in horses in a veterinary medical teaching hospital. J Vet Diagn Invest 7:343–346 [View Article][PubMed]
    [Google Scholar]
  18. Magdesian K. G., Hirsh D. C., Jang S. S., Hansen L. M., Madigan J. E. 2002; Characterization of Clostridium difficile isolates from foals with diarrhea: 28 cases (1993–1997). J Am Vet Med Assoc 220:67–73 [View Article][PubMed]
    [Google Scholar]
  19. Medina-Torres C. E., Weese J. S., Staempfli H. R. 2010; Validation of a commercial enzyme immunoassay for detection of Clostridium difficile toxins in feces of horses with acute diarrhea. J Vet Intern Med 24:628–632 [View Article][PubMed]
    [Google Scholar]
  20. Peláez T., Alcalá L., Alonso R., Rodríguez-Créixems M., García-Lechuz J. M., Bouza E. 2002; Reassessment of Clostridium difficile susceptibility to metronidazole and vancomycin. Antimicrob Agents Chemother 46:1647–1650 [View Article][PubMed]
    [Google Scholar]
  21. Riley T. V. 1998; Clostridium difficile: a pathogen of the nineties. Eur J Clin Microbiol Infect Dis 17:137–141[PubMed]
    [Google Scholar]
  22. Riley T. V. 2006; Epidemic Clostridium difficile . Med J Aust 185:133–134[PubMed]
    [Google Scholar]
  23. Riley T. V., Adams J. E., O’Neill G. L., Bowman R. A. 1991; Gastrointestinal carriage of Clostridium difficile in cats and dogs attending veterinary clinics. Epidemiol Infect 107:659–665 [View Article][PubMed]
    [Google Scholar]
  24. Rodriguez-Palacios A., Stämpfli H. R., Duffield T., Peregrine A. S., Trotz-Williams L. A., Arroyo L. G., Brazier J. S., Weese J. S. 2006; Clostridium difficile PCR ribotypes in calves, Canada. Emerg Infect Dis 12:1730–1736[PubMed] [CrossRef]
    [Google Scholar]
  25. Ruby R., Magdesian K. G., Kass P. H. 2009; Comparison of clinical, microbiologic, and clinicopathologic findings in horses positive and negative for Clostridium difficile infection. J Am Vet Med Assoc 234:777–784 [View Article][PubMed]
    [Google Scholar]
  26. Rupnik M. 2007; Is Clostridium difficile-associated infection a potentially zoonotic and foodborne disease?. Clin Microbiol Infect 13:457–459 [View Article][PubMed]
    [Google Scholar]
  27. Songer J. G., Post K. W., Larson D. J., Jost B. H., Glock R. D. 2000; Infection of neonatal swine with Clostridium difficile . Swine Health Prod 8:185–189[PubMed]
    [Google Scholar]
  28. Songer J. G., Trinh H. T., Dial S. M., Brazier J. S., Glock R. D. 2009a; Equine colitis X associated with infection by Clostridium difficile NAP1/027. J Vet Diagn Invest 21:377–380 [View Article][PubMed]
    [Google Scholar]
  29. Songer J. G., Trinh H. T., Killgore G. E., Thompson A. D., McDonald L. C., Limbago B. M. 2009b ). Clostridium difficile in retail meat products, USA, 2007. Emerg Infect Dis 15:819–821 [View Article][PubMed]
    [Google Scholar]
  30. Stubbs S. L., Brazier J. S., O’Neill G. L., Duerden B. I. 1999; PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes. J Clin Microbiol 37:461–463[PubMed]
    [Google Scholar]
  31. Weese J. S. 2002; A review of equine zoonotic diseases: risks in veterinary medicine. AAEP Proceedings 48:362–369
    [Google Scholar]
  32. Weese J. S., Staempfli H. R., Prescott J. F. 2000; Isolation of environmental Clostridium difficile from a veterinary teaching hospital. J Vet Diagn Invest 12:449–452 [View Article][PubMed]
    [Google Scholar]
  33. Weese J. S., Staempfli H. R., Prescott J. F. 2001; A prospective study of the roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in equine diarrhoea. Equine Vet J 33:403–409 [View Article][PubMed]
    [Google Scholar]
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