1887

Abstract

Surgical site infections are the second most common hospital- and community-acquired Gram-positive infections, with the US Centers for Disease Control and Prevention estimating that about 500 000 surgical site infections occur annually in the USA. The aim of this work was to determine the activity of the saponin diosgenyl 2-amino-2-deoxy-β--glucopyranoside hydrochloride (HSM1) and its bactericidal effect for a large number of Gram-positive cocci, as well as to investigate its interaction with seven clinically used antibiotics. , a wound model was established through the panniculus carnosus of BALB/c mice and then inoculated with 5×10 c.f.u. or . For each bacterial strain, the study included an infected or non-infected group that did not receive any treatment, a group treated with local HSM1, a group treated with intraperitoneal vancomycin, a group treated with intraperitoneal daptomycin and two groups that received HSM1 local treatment plus intraperitoneal vancomycin or daptomycin. All isolates were inhibited by HSM1 at concentrations of 2–32 mg l. Synergy was demonstrated when HSM1 was combined with vancomycin and daptomycin. In studies, all groups treated with single drugs showed a statistically significant result compared with the control group. The two groups treated with drug combinations showed the highest antimicrobial efficacy. The good activities and the efficacy suggest HSM1 as a promising therapeutic candidate in Gram-positive wound infections.

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2011-09-01
2024-03-29
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References

  1. Aremu A. O., Fawole O. A., Chukwujekwu J. C., Light M. E., Finnie J. F., Van Staden J. 2010; In vitro antimicrobial, anthelmintic and cyclooxygenase-inhibitory activities and phytochemical analysis of Leucosidea sericea. J Ethnopharmacol 131:22–27 [View Article][PubMed]
    [Google Scholar]
  2. Bouza E. 2009; New therapeutic choices for infections caused by methicillin-resistant Staphylococcus aureus. Clin Microbiol Infect 15:Suppl. 744–52 [View Article][PubMed]
    [Google Scholar]
  3. Bratzler D. W., Houck P. M.Surgical Infection Prevention Guidelines Writers Workgroup 2004; Antimicrobial prophylaxis for surgery: an advisory statement from the National Surgical Infection Prevention Project. Clin Infect Dis 38:1706–1715 [View Article][PubMed]
    [Google Scholar]
  4. Burke J. P. 2003; Infection control – a problem for patient safety. N Engl J Med 348:651–656 [View Article][PubMed]
    [Google Scholar]
  5. NCCLS 2003 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically Approved Standard. M7-A6 Villanova, PA: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  6. Cormican M. G., Jones R. N. 1996; Emerging resistance to antimicrobial agents in Gram-positive bacteria. Enterococci, staphylococci and nonpneumococcal streptococci. Drugs 51:Suppl. 16–12 [View Article][PubMed]
    [Google Scholar]
  7. Datta S., Maitra S., Gayen P., Sinha Babu S. P. 2009; Improved efficacy of tetracycline by acaciasides on Dirofilaria immitis. Parasitol Res 105:697–702 [View Article][PubMed]
    [Google Scholar]
  8. Di Liberto M., Svetaz L., Furlán R. L., Zacchino S. A., Delporte C., Novoa M. A., Asencio M., Cassels B. K. 2010; Antifungal activity of saponin-rich extracts of Phytolacca dioica and of the sapogenins obtained through hydrolysis. Nat Prod Commun 5:1013–1018[PubMed]
    [Google Scholar]
  9. Eliopoulos G. M., Moellering R. C. Jr 1996; Antimicrobial combinations. In Antibiotics in Laboratory Medicine pp. 330–393 Edited by Lorian V. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  10. Fisher K., Phillips C. 2009; The ecology, epidemiology and virulence of Enterococcus. Microbiology 155:1749–1757 [View Article][PubMed]
    [Google Scholar]
  11. Grundmann H., Aires-de-Sousa M., Boyce J., Tiemersma E. 2006; Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. Lancet 368:874–885 [View Article][PubMed]
    [Google Scholar]
  12. Hirsch T., Koerber A., Jacobsen F., Dissemond J., Steinau H. U., Gatermann S., Al-Benna S., Kesting M., Seipp H. M., Steinstraesser L. 2010; Evaluation of toxic side effects of clinically used skin antiseptics in vitro. J Surg Res 164:344–350 [View Article][PubMed]
    [Google Scholar]
  13. Kugelberg E., Norström T., Petersen T. K., Duvold T., Andersson D. I., Hughes D. 2005; Establishment of a superficial skin infection model in mice by using Staphylococcus aureus and Streptococcus pyogenes. Antimicrob Agents Chemother 49:3435–3441 [View Article][PubMed]
    [Google Scholar]
  14. Linden P. K. 1998; Clinical implications of nosocomial Gram-positive bacteremia and superimposed antimicrobial resistance. Am J Med 104:24S–33S [View Article][PubMed]
    [Google Scholar]
  15. Maviglia R., Nestorini R., Pennisi M. 2009; Role of old antibiotics in multidrug resistant bacterial infections. Curr Drug Targets 10:895–905 [View Article][PubMed]
    [Google Scholar]
  16. Moellering R. C. Jr 1998; Problems with antimicrobial resistance in Gram-positive cocci. Clin Infect Dis 26:1177–1178 [View Article][PubMed]
    [Google Scholar]
  17. Myszka H., Bednarczyk D., Najder M., Kaca W. 2003; Synthesis and induction of apoptosis in B cell chronic leukemia by diosgenyl 2-amino-2-deoxy-β-d-glucopyranoside hydrochloride and its derivatives. Carbohydr Res 338:133–141 [View Article][PubMed]
    [Google Scholar]
  18. Qi X., Bakht S., Qin B., Leggett M., Hemmings A., Mellon F., Eagles J., Werck-Reichhart D., Schaller H. et al. 2006; A different function for a member of an ancient and highly conserved cytochrome P450 family: from essential sterols to plant defense. Proc Natl Acad Sci U S A 103:18848–18853 [View Article][PubMed]
    [Google Scholar]
  19. Qiang H., Zhang C., Shi Z.-B., Yang H.-Q., Wang K.-Z. 2010; Protective effects and mechanism of Panax notoginseng saponins on oxidative stress-induced damage and apoptosis of rabbit bone marrow stromal cells. Chin J Integr Med 16:525–530 [View Article][PubMed]
    [Google Scholar]
  20. Raad I., Alrahwan A., Rolston K. 1998; Staphylococcus epidermidis: emerging resistance and need for alternative agents. Clin Infect Dis 26:1182–1187 [View Article][PubMed]
    [Google Scholar]
  21. Saleem M., Nazir M., Ali M. S., Hussain H., Lee Y. S., Riaz N., Jabbar A. 2010; Antimicrobial natural products: an update on future antibiotic drug candidates. Nat Prod Rep 27:238–254 [View Article][PubMed]
    [Google Scholar]
  22. Simonetti O., Cirioni O., Ghiselli R., Goteri G., Scalise A., Orlando F., Silvestri C., Riva A., Saba V. et al. 2008; RNAIII-inhibiting peptide enhances healing of wounds infected with methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 52:2205–2211 [View Article][PubMed]
    [Google Scholar]
  23. Simons V., Morrissey J. P., Latijnhouwers M., Csukai M., Cleaver A., Yarrow C., Osbourn A. 2006; Dual effects of plant steroidal alkaloids on Saccharomyces cerevisiae. Antimicrob Agents Chemother 50:2732–2740 [View Article][PubMed]
    [Google Scholar]
  24. Spellberg B., Guidos R., Gilbert D., Bradley J., Boucher H. W., Scheld W. M., Bartlett J. G., Edwards J. JrInfectious Diseases Society of America 2008; The epidemic of antibiotic-resistant infections: a call to action for the medical community from the Infectious Diseases Society of America. Clin Infect Dis 46:155–164 [View Article][PubMed]
    [Google Scholar]
  25. Sung W. S., Lee D. G. 2008; The combination effect of Korean red ginseng saponins with kanamycin and cefotaxime against methicillin-resistant Staphylococcus aureus. Biol Pharm Bull 31:1614–1617 [View Article][PubMed]
    [Google Scholar]
  26. Wallace R. J. 2004; Antimicrobial properties of plant secondary metabolites. Proc Nutr Soc 63:621–629 [View Article][PubMed]
    [Google Scholar]
  27. Yang C.-R., Zhang Y., Jacob M. R., Khan S. I., Zhang Y.-J., Li X.-C. 2006; Antifungal activity of C-27 steroidal saponins. Antimicrob Agents Chemother 50:1710–1714 [View Article][PubMed]
    [Google Scholar]
  28. Ying-Wan, Wu Y.-L., Feng X.-C., Lian L.-H., Jiang Y.-Z., Nan J.-X. 2010; The protective effects of total saponins from Ornithogalum saundersiae (Liliaceae) on acute hepatic failure induced by lipopolysaccharide and d-galactosamine in mice. J Ethnopharmacol 132:450–455 [View Article][PubMed]
    [Google Scholar]
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