1887

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Volume 60, Issue 9

Detailed comparison of and biofilms under different conditions and their susceptibility to caspofungin and anidulafungin Free

Abstract

biofilm development can be influenced by diverse factors such as substrate, culture medium, carbohydrate source and pH. We have analysed biofilm formation of SC5314 and ATCC 2001 wild-type strains in the presence of different media (RPMI 1640 versus YNB) and using different pH values (pH 5.6 or 7.0). We determined adhesion and biofilm formation on polystyrene, changes in the expression of adhesin genes during these processes and the susceptibility of mature biofilms to echinocandins. Biofilms formed on polystyrene by both species proved to be influenced strongly by the composition of the medium rather than pH. and formed thicker biofilms in RPMI 1640 medium, whereas in YNB medium, both species manifested adhesion rather than characteristic multilayer biofilm architecture. The stimulated biofilm formation in RPMI 1640 medium at pH 7.0 corroborated positively with increased expression of adhesin genes, essential to biofilm formation , including and in and in . The thicker biofilms grown in RPMI 1640 medium were more tolerant to caspofungin and anidulafungin than YNB-grown biofilms. We also observed that mature biofilms were less susceptible in RPMI 1640 medium to echinocandins than biofilms. Environmental conditions, i.e. medium and pH, can significantly affect not only biofilm architecture, but also the expression profile of several genes involved during the different stages of biofilm development. In addition, growth conditions may also influence the antifungal-susceptibility profile of fungal populations within biofilm structures. Therefore, before designing any experimental biofilm set-up, it is important to consider the potential influence of external environmental factors on biofilm development.

  • Received:
  • Accepted:
  • Published Online:
© 2011 SGM
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2011-09-01
2024-04-24
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Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin
J. Med. Microbiol. 60, 1261 (2011); https://doi.org/10.1099/jmm.0.032037-0
/content/journal/jmm/10.1099/jmm.0.032037-0
/content/journal/jmm/10.1099/jmm.0.032037-0
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