Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC β-lactamase
Roh, Kyoung Ho and Song, Wonkeun and Chung, Hae-Sun and Lee, Yang Soon and Yum, Jong Hwa and Yi, Ha Na and Chun, Jong Sik and Yong, Dongeun and Lee, Kyungwon and Chong, Yunsop,, 61, 94-100 (2012),
doi = https://doi.org/10.1099/jmm.0.032573-0,
publicationName = Microbiology Society,
issn = 0022-2615,
abstract= In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA–DNA hybridization. The genetic environment of the bla ACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla ACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla ACT-1 gene originated from the chromosome of E. hormaechei.,
language=,
type=