Comparison of plasmid-mediated quinolone resistance and extended-spectrum β-lactamases in third-generation cephalosporin-resistant Enterobacteriaceae from four Irish hospitals Walsh, Fiona and Rogers, Thomas R.,, 61, 142-147 (2012), doi = https://doi.org/10.1099/jmm.0.035246-0, publicationName = Microbiology Society, issn = 0022-2615, abstract= In this study, the frequency of extended-spectrum β-lactamases (ESBL) and plasmid-mediated quinolone resistance (PMQR) mechanisms were investigated in 206 clinical isolates of third-generation cephalosporin (3GC)-resistant Enterobacteriaceae in four hospitals in the Republic of Ireland. bla CTX-M-15 was the predominant ESBL gene. Of these 3GC resistant isolates, 54 % were also resistant to ciprofloxacin. Investigation of the PMQR mechanisms revealed that the aac(6′)Ib-cr gene predominated in fluoroquinolone-resistant (FQR) strains of Escherichia coli and Klebsiella pneumoniae, while the qnrA gene predominated in the FQR strains of Enterobacter. The bla CTX-M-15 gene was frequently identified with the aac(6′)Ib-cr gene but was not always on the same plasmid. The prevalence of the bla CTX-M-15 gene appeared to be hospital-dependent. The epidemiology of both ESBL-producing and PMQR strains within the four hospitals indicated that their prevalence is not due to the spread of these resistance genes between isolates from different hospitals., language=, type=