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Volume 61, Issue 4

Emergence of VIM-1-carbapenemase-producing in Tyrol, Austria Free

Abstract

The rapid emergence and dissemination of carbapenemase-producing species and other members of the poses a considerable threat to the care of hospitalized patients and to public health. In this study, isolates demonstrating decreased susceptibility to carbapenems detected at the Division of Hygiene and Medical Microbiology, Innsbruck Medical University, between January 2006 and December 2010 were tested for , , , and using a multiplex PCR with published primers. PFGE was performed to determine the genetic relatedness. In total, 33 isolates (28 and 5 ) were collected during the study period. From 2006 to 2009, between two and seven isolates were found per year. In 2010, a significant increase of carbapenem-resistant strains was observed ( = 12). The gene was detected in all 28 isolates of . Typing of by PFGE revealed three distinct clusters, the biggest of which contained 18 isolates. These findings demonstrate the emergence of VIM-1-producing in Tyrol, western Austria. The clonal relationship confirms the risk of spread of these organisms and their possible persistence over time.

  • Received:
  • Accepted:
  • Published Online:
© 2012 SGM
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2012-04-01
2024-05-03
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References

  1. Aschbacher R., Pagani L., Doumith M., Pike R., Woodford N., Spoladore G., Larcher C., Livermore D. M. 2011; Metallo-β-lactamases among Enterobacteriaceae from routine samples in an Italian tertiary-care hospital and long-term care facilities during 2008. Clin Microbiol Infect 17:181–189 [View Article][PubMed]
     [Google Scholar]
  2. Cobo J., Morosini M. I., Pintado V., Tato M., Samaranch N., Baquero F., Cantón R. 2008; Use of tigecycline for the treatment of prolonged bacteremia due to a multiresistant VIM-1 and SHV-12 β-lactamase-producing Klebsiella pneumoniae epidemic clone. Diagn Microbiol Infect Dis 60:319–322 [View Article][PubMed]
     [Google Scholar]
  3. Cohen Stuart J., Leverstein-Van Hall M. A. on behalf of members of the Dutch Working Party on the Detection of Highly Resistant Microorganisms 2010; Guideline for phenotypic screening and confirmation of carbapenemases in Enterobacteriaceae . Int J Antimicrob Agents 36:205–210 [View Article][PubMed]
     [Google Scholar]
  4. Cornaglia G., Akova M., Amicosante G., Cantón R., Cauda R., Docquier J. D., Edelstein M., Frère J. M., Fuzi M. other authors 2007; Metallo-β-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues. Int J Antimicrob Agents 29:380–388 [View Article][PubMed]
     [Google Scholar]
  5. Daikos G. L., Karabinis A., Paramythiotou E., Syriopoulou V. P., Kosmidis C., Avlami A., Gargalianos P., Tzanetou K., Petropoulou D., Malamou-Lada H. 2007; VIM-1-producing Klebsiella pneumoniae bloodstream infections: analysis of 28 cases. Int J Antimicrob Agents 29:471–473 [View Article][PubMed]
     [Google Scholar]
  6. Daikos G. L., Petrikkos P., Psichogiou M., Kosmidis C., Vryonis E., Skoutelis A., Georgousi K., Tzouvelekis L. S., Tassios P. T. other authors 2009; Prospective observational study of the impact of VIM-1 metallo-β-lactamase on the outcome of patients with Klebsiella pneumoniae bloodstream infections. Antimicrob Agents Chemother 53:1868–1873 [View Article][PubMed]
     [Google Scholar]
  7. Deshpande L. M., Jones R. N., Fritsche T. R., Sader H. S. 2006; Occurrence and characterization of carbapenemase-producing Enterobacteriaceae: report from the SENTRY Antimicrobial Surveillance Program (2000–2004). Microb Drug Resist 12:223–230 [View Article][PubMed]
     [Google Scholar]
  8. EUCAST 2011; Breakpoint tables for interpretation of MICs and zone diameters. European Committee on Antimicrobial Susceptibility Testing. Available online at http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Disk_test_documents/EUCAST_breakpoints_v1.1.pdf Last date accessed 19 September 2011
  9. Falcone M., Mezzatesta M. L., Perilli M., Forcella C., Giordano A., Cafiso V., Amicosante G., Stefani S., Venditti M. 2009; Infections with VIM-1 metallo-β-lactamase-producing Enterobacter cloacae and their correlation with clinical outcome. J Clin Microbiol 47:3514–3519 [View Article][PubMed]
     [Google Scholar]
  10. Gröbner S., Linke D., Schütz W., Fladerer C., Madlung J., Autenrieth I. B., Witte W., Pfeifer Y. 2009; Emergence of carbapenem-non-susceptible extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates at the university hospital of Tübingen, Germany. J Med Microbiol 58:912–922 [View Article][PubMed]
     [Google Scholar]
  11. Lee E. H., Nicolas M. H., Kitzis M. D., Pialoux G., Collatz E., Gutmann L. 1991; Association of two resistance mechanisms in a clinical isolate of Enterobacter cloacae with high-level resistance to imipenem. Antimicrob Agents Chemother 35:1093–1098[PubMed] [CrossRef]
     [Google Scholar]
  12. Levison M. E., Mailapur Y. V., Pradhan S. K., Jacoby G. A., Adams P., Emery C. L., May P. L., Pitsakis P. G. 2002; Regional occurrence of plasmid-mediated SHV-7, an extended-spectrum beta-lactamase, in Enterobacter cloacae in Philadelphia teaching hospitals. Clin Infect Dis 35:1551–1554 [View Article][PubMed]
     [Google Scholar]
  13. Livermore D. M., Warner M., Mushtaq S., Doumith M., Zhang J., Woodford N. 2011; What remains against carbapenem-resistant Enterobacteriaceae? Evaluation of chloramphenicol, ciprofloxacin, colistin, fosfomycin, minocycline, nitrofurantoin, temocillin and tigecycline. Int J Antimicrob Agents 37:415–419 [View Article][PubMed]
     [Google Scholar]
  14. Lomaestro B. M., Tobin E. H., Shang W., Gootz T. 2006; The spread of Klebsiella pneumoniae carbapenemase-producing K. pneumoniae to upstate New York. Clin Infect Dis 43:e26–e28 [View Article][PubMed]
     [Google Scholar]
  15. Mathers A. J., Cox H. L., Bonatti H., Kitchel B., Brassinga A. K., Wispelwey B., Sawyer R. G., Pruett T. L., Hazen K. C. other authors 2009; Fatal cross infection by carbapenem-resistant Klebsiella in two liver transplant recipients. Transpl Infect Dis 11:257–265 [View Article][PubMed]
     [Google Scholar]
  16. Meletis G., Tzampaz E., Sianou E., Tzavaras I., Sofianou D. 2011; Colistin heteroresistance in carbapenemase-producing Klebsiella pneumoniae . J Antimicrob Chemother 66:946–947 [View Article][PubMed]
     [Google Scholar]
  17. Paterson D. L., Bonomo R. A. 2005; Extended-spectrum β-lactamases: a clinical update. Clin Microbiol Rev 18:657–686 [View Article][PubMed]
     [Google Scholar]
  18. Poirel L., Al Maskari Z., Al Rashdi F., Bernabeu S., Nordmann P. 2011; NDM-1-producing Klebsiella pneumoniae isolated in the Sultanate of Oman. J Antimicrob Chemother 66:304–306 [View Article][PubMed]
     [Google Scholar]
  19. Psichogiou M., Tassios P. T., Avlamis A., Stefanou I., Kosmidis C., Platsouka E., Paniara O., Xanthaki A., Toutouza M. other authors 2008; Ongoing epidemic of bla VIM-1-positive Klebsiella pneumoniae in Athens, Greece: a prospective survey. J Antimicrob Chemother 61:59–63 [View Article][PubMed]
     [Google Scholar]
  20. Queenan A. M., Bush K. 2007; Carbapenemases: the versatile β-lactamases. Clin Microbiol Rev 20:440–458 [View Article][PubMed]
     [Google Scholar]
  21. Rasmussen B. A., Bush K., Keeney D., Yang Y., Hare R., O’Gara C., Medeiros A. A. 1996; Characterization of IMI-1 beta-lactamase, a class A carbapenem-hydrolyzing enzyme from Enterobacter cloacae . Antimicrob Agents Chemother 40:2080–2086[PubMed]
     [Google Scholar]
  22. Ribot E. M., Fair M. A., Gautom R., Cameron D. N., Hunter S. B., Swaminathan B., Barrett T. J. 2006; Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157 : H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis 3:59–67 [View Article][PubMed]
     [Google Scholar]
  23. Struelens M. J., Monnet D. L., Magiorakos A. P., Santos O'Connor F., Giesecke J. European NDM-1 Survey Participants 2010; New Delhi metallo-beta-lactamase 1-producing Enterobacteriaceae: emergence and response in Europe. Euro Surveill 15:pii:19716[PubMed]
     [Google Scholar]
  24. Tato M., Coque T. M., Ruíz-Garbajosa P., Pintado V., Cobo J., Sader H. S., Jones R. N., Baquero F., Cantón R. 2007; Complex clonal and plasmid epidemiology in the first outbreak of Enterobacteriaceae infection involving VIM-1 metallo-β-lactamase in Spain: toward endemicity?. Clin Infect Dis 45:1171–1178 [View Article][PubMed]
     [Google Scholar]
  25. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239[PubMed]
     [Google Scholar]
  26. Treviño M., Moldes L., Martínez-Lamas L., Varón C., Regueiro B. J. 2009; Carbapenem-resistant Enterobacter cloacae and the emergence of metallo-β-lactamase-producing strains in a third-level hospital (Santiago de Compostela, NW Spain). Eur J Clin Microbiol Infect Dis 28:1253–1258 [View Article][PubMed]
     [Google Scholar]
  27. Tzelepi E., Giakkoupi P., Sofianou D., Loukova V., Kemeroglou A., Tsakris A. 2000; Detection of extended-spectrum beta-lactamases in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes . J Clin Microbiol 38:542–546[PubMed]
     [Google Scholar]
  28. Vatopoulos A. 2008; High rates of metallo-beta-lactamase-producing Klebsiella pneumoniae in Greece – a review of the current evidence. Euro Surveill 13:13[PubMed]
     [Google Scholar]
  29. Walsh T. R., Toleman M. A., Poirel L., Nordmann P. 2005; Metallo-β-lactamases: the quiet before the storm?. Clin Microbiol Rev 18:306–325 [View Article][PubMed]
     [Google Scholar]
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Emergence of VIM-1-carbapenemase-producing Enterobacter cloacae in Tyrol, Austria
J. Med. Microbiol. 61, 567 (2012); https://doi.org/10.1099/jmm.0.038646-0
/content/journal/jmm/10.1099/jmm.0.038646-0
/content/journal/jmm/10.1099/jmm.0.038646-0
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