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Volume 61, Issue 5

Evaluation of gene variation in (Lancefield group A ) and potential for subtyping Free

Abstract

Variation of gene sequences in group A (GAS) strains was evaluated for its potential use in subtyping the most common serotypes of encountered in the UK. We sequenced a total of 188 strains, including randomly selected invasive GAS and outbreak-related strains. Variability was highest amongst M/ 89 strains, whereas very little variation was observed amongst M/ 1 and M/ 28 GAS strains. Repeat patterns were identified in the collagen structure motif (CSM) of the M/ 89 GAS strains. The sporadic strains were very diverse and encompassed most of the CSM patterns, whereas the outbreak-related strains were mainly clustered into two CSM groups. gene cluster analysis distinguished outbreak strains from two different healthcare settings in the same geographical area. Sequence variations were assessed by the number of pentameric repeats (CAAAA) present at the 5′ region of the gene. The determination of polymorphisms amongst GAS serotype M/ 89 strains could be used as an important epidemiological marker to inform clinicians and outbreak control teams during outbreak investigations.

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© 2012 Health Protection Agency
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2012-05-01
2024-04-18
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References

  1. Agüero J., Ortega-Mendi M., Eliecer Cano M., Gonzalez de Aledo A., Calvo J., Viloria L., Mellado P., Pelayo T., Fernandez-Rodriguez A., Martinez-Martinez L. 2008; Outbreak of invasive group A streptococcal disease among children attending a day-care center. Pediatr Infect Dis J 27:602–604 [View Article][PubMed]
     [Google Scholar]
  2. Åkesson P., Rasmussen M., Mascini E., von Pawel-Rammingen U., Janulczyk R., Collin M., Olsen A., Mattsson E., Olsson M. L. other authors 2004; Low antibody levels against cell wall-attached proteins of Streptococcus pyogenes predispose for severe invasive disease. J Infect Dis 189:797–804 [View Article][PubMed]
     [Google Scholar]
  3. Aziz R. K., Kotb M. 2008; Rise and persistence of global M1T1 clone of Streptococcus pyogenes. Emerg Infect Dis 14:1511–1517 [View Article][PubMed]
     [Google Scholar]
  4. Bahnan W., Hashwa F., Araj G., Tokajian S. 2011; emm typing, antibiotic resistance and PFGE analysis of Streptococcus pyogenes in Lebanon. J Med Microbiol 60:98–101 [View Article][PubMed]
     [Google Scholar]
  5. Beall B., Facklam R., Thompson T. 1996; Sequencing emm-specific PCR products for routine and accurate typing of group A streptococci. J Clin Microbiol 34:953–958[PubMed]
     [Google Scholar]
  6. Beall B., Facklam R. R., Elliott J. A., Franklin A. R., Hoenes T., Jackson D., Laclaire L., Thompson T., Viswanathan R. 1998; Streptococcal emm types associated with T-agglutination types and the use of conserved emm gene restriction fragment patterns for subtyping group A streptococci. J Med Microbiol 47:893–898 [View Article][PubMed]
     [Google Scholar]
  7. Bidet P., Lesteven E., Doit C., Liguori S., Mariani-Kurkdjian P., Bonacorsi S., Bingen E. 2009; Subtyping of emm1 group A streptococci causing invasive infections in France. J Clin Microbiol 47:4146–4149 [View Article][PubMed]
     [Google Scholar]
  8. Carapetis J. R., Steer A. C., Mulholland E. K., Weber M. 2005; The global burden of group A streptococcal diseases. Lancet Infect Dis 5:685–694 [View Article][PubMed]
     [Google Scholar]
  9. Carriço J. A., Silva-Costa C., Melo-Cristino J., Pinto F. R., de Lencastre H., Almeida J. S., Ramirez M. 2006; Illustration of a common framework for relating multiple typing methods by application to macrolide-resistant Streptococcus pyogenes. J Clin Microbiol 44:2524–2532 [View Article][PubMed]
     [Google Scholar]
  10. Caswell C. C., Han R., Hovis K. M., Ciborowski P., Keene D. R., Marconi R. T., Lukomski S. 2008; The Scl1 protein of M6-type group A Streptococcus binds the human complement regulatory protein, factor H, and inhibits the alternative pathway of complement. Mol Microbiol 67:584–596 [View Article][PubMed]
     [Google Scholar]
  11. Caswell C. C., Oliver-Kozup H., Han R., Lukomska E., Lukomski S. 2010; Scl1, the multifunctional adhesin of group A Streptococcus, selectively binds cellular fibronectin and laminin, and mediates pathogen internalization by human cells. FEMS Microbiol Lett 303:61–68 [View Article][PubMed]
     [Google Scholar]
  12. Coelho J. M., De Zoysa A., Daniel R., Dhami C., Lamagni T. L., George R., Efstratiou A. 2010; Microbiological characterisation of group A streptococcal isolates from an invasive disease upsurge in England. In: 2010 European Scientific Conference on Applied Infectious Disease Epidemiology (ESCAIDE) edn, p. 118
    [Google Scholar]
  13. Enright M. C., Spratt B. G., Kalia A., Cross J. H., Bessen D. E. 2001; Multilocus sequence typing of Streptococcus pyogenes and the relationships between emm type and clone. Infect Immun 69:2416–2427 [View Article][PubMed]
     [Google Scholar]
  14. Graham M. R., Virtaneva K., Porcella S. F., Gardner D. J., Long R. D., Welty D. M., Barry W. T., Johnson C. A., Parkins L. D. other authors 2006; Analysis of the transcriptome of group A Streptococcus in mouse soft tissue infection. Am J Pathol 169:927–942 [View Article][PubMed]
     [Google Scholar]
  15. Hoe N., Nakashima K., Grigsby D., Pan X., Dou S. J., Naidich S., Garcia M., Kahn E., Bergmire-Sweat D., Musser J. M. 1999; Rapid molecular genetic subtyping of serotype M1 group A Streptococcus strains. Emerg Infect Dis 5:254–263 [View Article][PubMed]
     [Google Scholar]
  16. Imöhl M., Reinert R. R., Ocklenburg C., van der Linden M. 2010; Epidemiology of invasive Streptococcus pyogenes disease in Germany during 2003–2007. FEMS Immunol Med Microbiol 58:389–396[PubMed]
     [Google Scholar]
  17. Johnson D. R., Kaplan E. L., Sramek J., Bicova R., Havlicek J., Havlickova H., Motlova J., Kriz P. 1996; Determination of T-protein agglutination patterns. In Laboratory Diagnosis of Group A Streptococcal Infections pp. 37–41 Edited by Johnson D. R., Kaplan E. L., Sramek J., Bicova R., Havlicek J., Havlickova H., Motlova J., Kriz P. Geneva, Switzerland: World Health Organization;
     [Google Scholar]
  18. Johnson D. R., Kaplan E. L., VanGheem A., Facklam R. R., Beall B. 2006; Characterization of group A streptococci (Streptococcus pyogenes): correlation of M-protein and emm-gene type with T-protein agglutination pattern and serum opacity factor. J Med Microbiol 55:157–164 [View Article][PubMed]
     [Google Scholar]
  19. Junier T., Pagni M. 2000; Dotlet: diagonal plots in a web browser. Bioinformatics 16:178–179 [View Article][PubMed]
     [Google Scholar]
  20. Lacy M. D., Horn K. 2009; Nosocomial transmission of invasive group A Streptococcus from patient to health care worker. Clin Infect Dis 49:354–357 [View Article][PubMed]
     [Google Scholar]
  21. Lamagni T. L., Neal S., Keshishian C., Alhaddad N., George R., Duckworth G., Vuopio-Varkila J., Efstratiou A. 2008; Severe Streptococcus pyogenes infections, United Kingdom, 2003–2004. Emerg Infect Dis 14:202–209 [View Article][PubMed]
     [Google Scholar]
  22. Lamagni T. L., Efstratiou A., Dennis J., Nair P., Kearney J., George R.on behalf of the national incident management team 2009; Increase in invasive group A streptococcal infections in England, Wales and Northern Ireland, 2008–9. Euro Surveill 14:pii = 19110 Available online http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19110.[PubMed]
     [Google Scholar]
  23. Lukomski S., Nakashima K., Abdi I., Cipriano V. J., Ireland R. M., Reid S. D., Adams G. G., Musser J. M. 2000; Identification and characterization of the scl gene encoding a group A Streptococcus extracellular protein virulence factor with similarity to human collagen. Infect Immun 68:6542–6553 [View Article][PubMed]
     [Google Scholar]
  24. Lukomski S., Nakashima K., Abdi I., Cipriano V. J., Shelvin B. J., Graviss E. A., Musser J. M. 2001; Identification and characterization of a second extracellular collagen-like protein made by group A Streptococcus: control of production at the level of translation. Infect Immun 69:1729–1738 [View Article][PubMed]
     [Google Scholar]
  25. McGregor K. F., Spratt B. G. 2005; Identity and prevalence of multilocus sequence typing-defined clones of group A streptococci within a hospital setting. J Clin Microbiol 43:1963–1967 [View Article][PubMed]
     [Google Scholar]
  26. Meisal R., Andreasson I. K., Høiby E. A., Aaberge I. S., Michaelsen T. E., Caugant D. A. 2010a; Streptococcus pyogenes isolates causing severe infections in Norway in 2006 to 2007: emm types, multilocus sequence types, and superantigen profiles. J Clin Microbiol 48:842–851 [View Article][PubMed]
     [Google Scholar]
  27. Meisal R., Høiby E. A., Caugant D. A., Musser J. M. 2010b; Molecular characteristics of pharyngeal and invasive emm3 Streptococcus pyogenes strains from Norway, 1988–2003. Eur J Clin Microbiol Infect Dis 29:31–43 [View Article][PubMed]
     [Google Scholar]
  28. Mohs A., Silva T., Yoshida T., Amin R., Lukomski S., Inouye M., Brodsky B. 2007; Mechanism of stabilization of a bacterial collagen triple helix in the absence of hydroxyproline. J Biol Chem 282:29757–29765 [View Article][PubMed]
     [Google Scholar]
  29. Mora M., Bensi G., Capo S., Falugi F., Zingaretti C., Manetti A. G., Maggi T., Taddei A. R., Grandi G., Telford J. L. 2005; Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens. Proc Natl Acad Sci U S A 102:15641–15646 [View Article][PubMed]
     [Google Scholar]
  30. Neal S., Beall B., Ekelund K., Henriques-Normark B., Jasir A., Johnson D., Kaplan E., Lovgren M., Reinert R. R. other authors 2007; International quality assurance study for characterization of Streptococcus pyogenes. J Clin Microbiol 45:1175–1179 [View Article][PubMed]
     [Google Scholar]
  31. Podbielski A., Melzer B., Lütticken R. 1991; Application of the polymerase chain reaction to study the M protein(-like) gene family in β-hemolytic streptococci. Med Microbiol Immunol (Berl) 180:213–227 [View Article][PubMed]
     [Google Scholar]
  32. Rasmussen M., Björck L. 2001; Unique regulation of SclB – a novel collagen-like surface protein of Streptococcus pyogenes. Mol Microbiol 40:1427–1438 [View Article][PubMed]
     [Google Scholar]
  33. Rasmussen M., Edén A., Björck L. 2000; SclA, a novel collagen-like surface protein of Streptococcus pyogenes. Infect Immun 68:6370–6377 [View Article][PubMed]
     [Google Scholar]
  34. Sakota V., Fry A. M., Lietman T. M., Facklam R. R., Li Z., Beall B. 2006; Genetically diverse group A streptococci from children in far-western Nepal share high genetic relatedness with isolates from other countries. J Clin Microbiol 44:2160–2166 [View Article][PubMed]
     [Google Scholar]
  35. Siljander T., Lyytikäinen O., Vähäkuopus S., Snellman M., Jalava J., Vuopio J. 2010; Epidemiology, outcome and emm types of invasive group A streptococcal infections in Finland. Eur J Clin Microbiol Infect Dis 29:1229–1235 [View Article][PubMed]
     [Google Scholar]
  36. Silva-Costa C., Pinto F. R., Ramirez M., Melo-Cristino J.The Portuguese Surveillance Group for the Study of Respiratory Pathogens 2008; Decrease in macrolide resistance and clonal instability among Streptococcus pyogenes in Portugal. Clin Microbiol Infect 14:1152–1159 [View Article][PubMed]
     [Google Scholar]
  37. Steer A. C., Jenney A. W., Kado J., Batzloff M. R., La Vincente S., Waqatakirewa L., Mulholland E. K., Carapetis J. R. 2009a; High burden of impetigo and scabies in a tropical country. PLoS Negl Trop Dis 3:e467 [View Article][PubMed]
     [Google Scholar]
  38. Steer A. C., Law I., Matatolu L., Beall B. W., Carapetis J. R. 2009b; Global emm type distribution of group A streptococci: systematic review and implications for vaccine development. Lancet Infect Dis 9:611–616 [View Article][PubMed]
     [Google Scholar]
  39. Takayama Y., Hikawa S., Okada J., Sunakawa K., Akahoshi T. 2009; A foodborne outbreak of a group A streptococcal infection in a Japanese university hospital. Eur J Clin Microbiol Infect Dis 28:305–308 [View Article][PubMed]
     [Google Scholar]
  40. Thigpen M. C., Thomas D. M., Gloss D., Park S. Y., Khan A. J., Fogelman V. L., Beall B., Van Beneden C. A., Todd R. L., Greene C. M. 2007; Nursing home outbreak of invasive group a streptococcal infections caused by 2 distinct strains. Infect Control Hosp Epidemiol 28:68–74[PubMed]
     [Google Scholar]
  41. Vikerfors A., Haggar A., Darenberg J., Low A., Melhus A., Hedlund J., Sylvan S., Norrby-Teglund A., Eriksson B. M. 2009; Severe group A streptococcal infections in Uppsala County, Sweden: clinical and molecular characterization of a case cluster from 2006 to 2007. Scand J Infect Dis 41:823–830 [View Article][PubMed]
     [Google Scholar]
  42. Wahl R. U., Lütticken R., Stanzel S., van der Linden M., Reinert R. R. 2007; Epidemiology of invasive Streptococcus pyogenes infections in Germany, 1996–2002: results from a voluntary laboratory surveillance system. Clin Microbiol Infect 13:1173–1178 [View Article][PubMed]
     [Google Scholar]
  43. Whatmore A. M. 2001; Streptococcus pyogenes sclB encodes a putative hypervariable surface protein with a collagen-like repetitive structure. Microbiology 147:419–429[PubMed]
     [Google Scholar]
  44. Xu Y., Keene D. R., Bujnicki J. M., Höök M., Lukomski S. 2002; Streptococcal Scl1 and Scl2 proteins form collagen-like triple helices. J Biol Chem 277:27312–27318 [View Article][PubMed]
     [Google Scholar]
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Evaluation of sclB gene variation in Streptococcus pyogenes (Lancefield group A Streptococcus) and potential for subtyping
J. Med. Microbiol. 61, 615 (2012); https://doi.org/10.1099/jmm.0.040535-0
/content/journal/jmm/10.1099/jmm.0.040535-0
/content/journal/jmm/10.1099/jmm.0.040535-0
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