1887

Abstract

A real-time PCR assay targeting the gene was developed to detect [Lancefield group B (GBS)] from clinical samples. A total of 110 blood culture-negative samples [75 cerebrospinal fluid (CSF) and 35 EDTA blood samples] from neonates with probable GBS sepsis or meningitis were analysed. Among these, 16 of the 75 CSF samples were positive [21.3 %, 95 % confidence interval (CI) 12.7–32.3 %] and two of the 35 EDTA blood samples were positive (5.7 %, 95 % CI 0.7–19.2 %). The proportion testing positive in the CSF samples was significantly higher than in the EDTA blood samples ( = 0.05, Fisher’s exact test). Overall, this real-time PCR assay was shown to be superior to culture methods for detection of GBS from CSF and EDTA blood samples.

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2012-08-01
2024-03-29
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References

  1. Al-Soud W. A., Rådström P. 2001; Purification and characterization of PCR-inhibitory components in blood cells. J Clin Microbiol 39:485–493 [View Article][PubMed]
    [Google Scholar]
  2. Atkins K. L., Atkinson R. M., Shanks A., Parvin C. A., Dunne W. M., Gross G. 2006; Evaluation of polymerase chain reaction for group B Streptococcus detection using an improved culture method. Obstet Gynecol 108:488–491 [View Article][PubMed]
    [Google Scholar]
  3. Bergh K., Stoelhaug A., Loeseth K., Bevanger L. 2004; Detection of group B streptococci (GBS) in vaginal swabs using real-time PCR with TaqMan probe hybridization. Indian J Med Res 119:Suppl.221–223[PubMed]
    [Google Scholar]
  4. Bisharat N., Jones N., Marchaim D., Block C., Harding R. M., Yagupsky P., Peto T., Crook D. W. 2005; Population structure of group B streptococcus from a low-incidence region for invasive neonatal disease. Microbiology 151:1875–1881 [View Article][PubMed]
    [Google Scholar]
  5. Bliss S. J., Manning S. D., Tallman P., Baker C. J., Pearlman M. D., Marrs C. F., Foxman B. 2002; Group B streptococcus colonization in male and nonpregnant female university students: a cross-sectional prevalence study. Clin Infect Dis 34:184–190 [View Article][PubMed]
    [Google Scholar]
  6. Bohnsack J. F., Whiting A., Gottschalk M., Dunn D. M., Weiss R., Azimi P. H., Philips J. B. III, Weisman L. E., Rhoads G. G., Lin F.-Y. C. 2008; Population structure of invasive and colonizing strains of Streptococcus agalactiae from neonates of six US academic centers from 1995 to 1999. J Clin Microbiol 46:1285–1291 [View Article][PubMed]
    [Google Scholar]
  7. Dogan B., Schukken Y. H., Santisteban C., Boor K. J. 2005; Distribution of serotypes and antimicrobial resistance genes among Streptococcus agalactiae isolates from bovine and human hosts. J Clin Microbiol 43:5899–5906 [View Article][PubMed]
    [Google Scholar]
  8. Edmond K. M., Kortsalioudaki C., Scott S., Schrag S. J., Zaidi A. K. M., Cousens S., Heath P. T. 2012; Group B streptococcal disease in infants aged younger than 3 months: systematic review and meta-analysis. Lancet 379:547–556 [View Article][PubMed]
    [Google Scholar]
  9. Edwards R. K., Novak-Weekley S. M., Koty P. P., Davis T., Leeds L. J., Jordan J. A. 2008; Rapid group B streptococci screening using a real-time polymerase chain reaction assay. Obstet Gynecol 111:1335–1341 [View Article][PubMed]
    [Google Scholar]
  10. Ippolito D. L., James W. A., Tinnemore D., Huang R. R., Dehart M. J., Williams J., Wingerd M. A., Demons S. T. 2010; Group B streptococcus serotype prevalence in reproductive-age women at a tertiary care military medical center relative to global serotype distribution. BMC Infect Dis 10:336 [View Article][PubMed]
    [Google Scholar]
  11. Jones N., Bohnsack J. F., Takahashi S., Oliver K. A., Chan M.-S., Kunst F., Glaser P., Rusniok C., Crook D. W. M. other authors 2003; Multilocus sequence typing system for group B streptococcus. J Clin Microbiol 41:2530–2536 [View Article][PubMed]
    [Google Scholar]
  12. Jones N., Oliver K. A., Barry J., Harding R. M., Bisharat N., Spratt B. G., Peto T., Crook D. W. M. Oxford Group B Streptococcus Consortium 2006; Enhanced invasiveness of bovine-derived neonatal sequence type 17 group B Streptococcus is independent of capsular serotype. Clin Infect Dis 42:915–924 [View Article][PubMed]
    [Google Scholar]
  13. Ke D., Ménard C., Picard F. J., Boissinot M., Ouellette M., Roy P. H., Bergeron M. G. 2000; Development of conventional and real-time PCR assays for the rapid detection of group B streptococci. Clin Chem 46:324–331[PubMed]
    [Google Scholar]
  14. Lin F.-Y. C., Whiting A., Adderson E., Takahashi S., Dunn D. M., Weiss R., Azimi P. H., Philips J. B. III, Weisman L. E. other authors 2006; Phylogenetic lineages of invasive and colonizing strains of serotype III group B streptococci from neonates: a multicenter prospective study. J Clin Microbiol 44:1257–1261 [View Article][PubMed]
    [Google Scholar]
  15. Luan S. L., Granlund M., Sellin M., Lagergård T., Spratt B. G., Norgren M. 2005; Multilocus sequence typing of Swedish invasive group B streptococcus isolates indicates a neonatally associated genetic lineage and capsule switching. J Clin Microbiol 43:3727–3733 [View Article][PubMed]
    [Google Scholar]
  16. Madzivhandila M., Adrian P. V., Cutland C. L., Kuwanda L., Schrag S. J., Madhi S. A. 2011; Serotype distribution and invasive potential of group B streptococcus isolates causing disease in infants and colonizing maternal-newborn dyads. PLoS ONE 6:e17861 [View Article][PubMed]
    [Google Scholar]
  17. Melin P. 2011; Neonatal group B streptococcal disease: from pathogenesis to preventive strategies. Clin Microbiol Infect 17:1294–1303[PubMed] [CrossRef]
    [Google Scholar]
  18. Musser J. M., Mattingly S. J., Quentin R., Goudeau A., Selander R. K. 1989; Identification of a high-virulence clone of type III Streptococcus agalactiae (group B Streptococcus) causing invasive neonatal disease. Proc Natl Acad Sci U S A 86:4731–4735 [View Article][PubMed]
    [Google Scholar]
  19. Pettersson K. 2007; Perinatal infection with group B streptococci. Semin Fetal Neonatal Med 12:193–197 [View Article][PubMed]
    [Google Scholar]
  20. Riedlinger J., Beqaj S. H., Milish M. A., Young S., Smith R., Dodd M., Hankerd R. E., Lebar W. D., Newton D. W. 2010; Multicenter evaluation of the BD Max GBS assay for detection of group B streptococci in prenatal vaginal and rectal screening swab specimens from pregnant women. J Clin Microbiol 48:4239–4241 [View Article][PubMed]
    [Google Scholar]
  21. Slotved H. C., Kong F., Lambertsen L., Sauer S., Gilbert G. L. 2007; Serotype IX, a proposed new Streptococcus agalactiae serotype. J Clin Microbiol 45:2929–2936 [View Article][PubMed]
    [Google Scholar]
  22. Spellerberg B., Pohl B., Haase G., Martin S., Weber-Heynemann J., Lütticken R. 1999; Identification of genetic determinants for the hemolytic activity of Streptococcus agalactiae by ISS1 transposition. J Bacteriol 181:3212–3219[PubMed]
    [Google Scholar]
  23. Trijbels-Smeulders M. A., Kollée L. A., Adriaanse A. H., Kimpen J. L., Gerards L. J. 2004; Neonatal group B streptococcal infection: incidence and strategies for prevention in Europe. Pediatr Infect Dis J 23:172–173[PubMed] [CrossRef]
    [Google Scholar]
  24. Verani J. R., Schrag S. J. 2010; Group B streptococcal disease in infants: progress in prevention and continued challenges. Clin Perinatol 37:375–392 [View Article][PubMed]
    [Google Scholar]
  25. Wernecke M., Mullen C., Sharma V., Morrison J., Barry T., Maher M., Smith T. 2009; Evaluation of a novel real-time PCR test based on the ssrA gene for the identification of group B streptococci in vaginal swabs. BMC Infect Dis 9:148 [View Article][PubMed]
    [Google Scholar]
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