RT Journal Article SR Electronic(1) A1 Liu, Bao-Tao A1 Liao, Xiao-Ping A1 Yang, Shou-Shen A1 Wang, Xiu-Mei A1 Li, Lu-Lu A1 Sun, Jian A1 Yang, Yu-Rong A1 Fang, Liang-Xing A1 Li, Liang A1 Zhao, Dong-Hao A1 Liu, Ya-HongYR 2012 T1 Detection of mutations in the gyrA and parC genes in Escherichia coli isolates carrying plasmid-mediated quinolone resistance genes from diseased food-producing animals JF Journal of Medical Microbiology, VO 61 IS 11 SP 1591 OP 1599 DO https://doi.org/10.1099/jmm.0.043307-0 PB Microbiology Society, SN 1473-5644, AB In this study, the prevalence of plasmid-mediated quinolone resistance (PMQR) was investigated in 495 Escherichia coli isolates from diseased food-producing animals in Guangdong province, China. The quinolone resistance-determining regions (QRDRs) of the gyrA and parC genes were analysed for mutations in 55 isolates harbouring only oqxAB and all isolates harbouring other PMQR genes. Overall, 282 (57.0 %) E. coli isolates had at least one PMQR gene. oqxAB was detected in 215 isolates and predominated the PMQR genes, followed by qnrS (63 isolates), aac(6′)-Ib-cr (56 isolates), qnrB (39 isolates) and qepA (18 isolates). qnrA, qnrC and qnrD were not found in any of the isolates. The rates of resistance to ciprofloxacin, enrofloxacin, levofloxacin and nalidixic acid were 75.2, 81.0, 70.5 and 97.4 %, respectively, among the 495 isolates. Eight types of mutation in gyrA were detected in 154 PMQR-positive isolates, and 147 isolates were found to have mutations in parC. PFGE analysis indicated that the PMQR-positive E. coli isolates were genetically diverse. This study demonstrated that the number of mutations in QRDRs of gyrA and/or parC was significantly associated with the MICs of quinolones (P<0.01). The rates of resistance to ciprofloxacin, enrofloxacin and nalidixic acid in PMQR-positive isolates were significantly higher than those in PMQR-negative isolates (P<0.05). In addition, the prevalence of oqxAB had significant Spearman correlation coefficients in relation to the MICs of all four tested quinolones (P<0.01)., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.043307-0