1887

Abstract

In this study, nonchromogenic mycobacteria were isolated from pulmonary samples of three patients in the Netherlands. All isolates had identical, unique 16S rRNA gene and 16S–23S ITS sequences, which were closely related to those of and . The biochemical features of the isolates differed slightly from those of , suggesting that the isolates may represent a possible separate species within the complex (MAC). However, the cell-wall mycolic acid pattern, analysed by HPLC, and the partial sequences of the and genes were identical to those of . We concluded that the isolates represent a novel variant of . The results of this analysis have led us to question the currently used methods of species definition for members of the genus , which are based largely on 16S rRNA or gene sequencing. Definitions based on a single genetic target are likely to be insufficient. Genetic divergence, especially in the MAC, yields strains that cannot be confidently assigned to a specific species based on the analysis of a single genetic target.

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2012-09-01
2024-03-28
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