1887

Abstract

We screened 21 extended spectrum β-lactamase-producing with reduced susceptibility to carbapenems for carbapenemase production. Five strains (four and one ) showed carbapenemase production, which was identified as OXA-48. The gene was detected on ~54 kb plasmids belonging to IncA/C in one case. Two isolates harboured IS, which is involved in mobilization. Carbapenem resistance in enterobacteria should be regarded as an emerging clinical problem in our hospital and necessitates rigorous surveillance in order to limit its spread.

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2012-12-01
2024-04-18
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References

  1. Carattoli A., Bertini A., Villa L., Falbo V., Hopkins K. L., Threlfall E. J. 2005; Identification of plasmids by PCR-based replicon typing. J Microbiol Methods 63:219–228 [View Article][PubMed]
    [Google Scholar]
  2. Carrër A., Poirel L., Yilmaz M., Akan O. A., Feriha C., Cuzon G., Matar G., Honderlick P., Nordmann P. 2010; Spread of OXA-48-encoding plasmid in Turkey and beyond. Antimicrob Agents Chemother 54:1369–1373 [View Article][PubMed]
    [Google Scholar]
  3. CLSI 2011 Performance Standards for Antimicrobial Susceptibility Testing, Twenty-first informational supplement, M100–S21 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  4. Cohen Stuart J., Leverstein-Van Hall M. A.Dutch Working Party on the Detection of Highly Resistant Microorganisms 2010; Guideline for phenotypic screening and confirmation of carbapenemases in Enterobacteriaceae. Int J Antimicrob Agents 36:205–210 [View Article][PubMed]
    [Google Scholar]
  5. Cuzon G., Naas T., Lesenne A., Benhamou M., Nordmann P. 2010; Plasmid-mediated carbapenem-hydrolysing OXA-48 β-lactamase in Klebsiella pneumoniae from Tunisia. Int J Antimicrob Agents 36:91–93 [View Article][PubMed]
    [Google Scholar]
  6. Gaul S. B., Wedel S., Erdman M. M., Harris D. L., Harris I. T., Ferris K. E., Hoffman L. 2007; Use of pulsed-field gel electrophoresis of conserved XbaI fragments for identification of swine Salmonella serotypes. J Clin Microbiol 45:472–476 [View Article][PubMed]
    [Google Scholar]
  7. Glupczynski Y., Huang T. D., Bouchahrouf W., Rezende de Castro R., Bauraing C., Gérard M., Verbruggen A. M., Deplano A., Denis O., Bogaerts P. 2012; Rapid emergence and spread of OXA-48-producing carbapenem-resistant Enterobacteriaceae isolates in Belgian hospitals. Int J Antimicrob Agents 39:168–172 [View Article][PubMed]
    [Google Scholar]
  8. Ktari S., Mnif B., Louati F., Rekik S., Mezghani S., Mahjoubi F., Hammami A. 2011; Spread of Klebsiella pneumoniae isolates producing OXA-48 β-lactamase in a Tunisian university hospital. J Antimicrob Chemother 66:1644–1646 [View Article][PubMed]
    [Google Scholar]
  9. Lee K., Chong Y., Shin H. B., Kim Y. A., Yong D., Yum J. H. 2001; Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of Pseudomonas and Acinetobacter species. Clin Microbiol Infect 7:88–91 [View Article][PubMed]
    [Google Scholar]
  10. Levast M., Poirel L., Carrër A., Deiber M., Decroisette E., Mallaval F. O., Lecomte C., Nordmann P. 2011; Transfer of OXA-48-positive carbapenem-resistant Klebsiella pneumoniae from Turkey to France. J Antimicrob Chemother 66:944–945 [View Article][PubMed]
    [Google Scholar]
  11. Poirel L., Héritier C., Tolün V., Nordmann P. 2004; Emergence of oxacillinase-mediated resistance to imipenem in Klebsiella pneumoniae. Antimicrob Agents Chemother 48:15–22 [View Article][PubMed]
    [Google Scholar]
  12. Poirel L., Bonnin R. A., Nordmann P. 2012; Genetic features of the widespread plasmid coding for the carbapenemase OXA-48. Antimicrob Agents Chemother 56:559–562 [View Article][PubMed]
    [Google Scholar]
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