1887

Abstract

Our previous studies have suggested that L-forms are able to pass through the placental barrier of mice from the maternal side to the fetal body and affect fetal growth and development, but little is known about the direct influence of L-forms on embryos during the critical period of organogenesis. Mouse embryos at gestational day 8.5 were cultured for 48 h with 0, 50, 100, 200 or 400 c.f.u. L-forms ml. At the end of the culture period, the mouse embryos were assessed morphologically for viability, growth and development. Bacteriological and immunohistochemical staining were used to determine the existence of L-forms in embryonic tissues. We found that both crown–rump length and head length of mouse embryos exposed to L-forms at a concentration of 50 c.f.u. ml were reduced. When the mouse embryos were exposed to 100, 200 or 400 c.f.u. L-forms ml, the total morphological score, number of somites, dry embryo weight, yolk sac diameter, crown–rump length and head length were significantly lower than those of the control group. With the increased concentration of L-forms in the culture medium, there were fewer normally developed embryos and more embryos with abnormalities or retardation in growth. L-forms detected by Gram-staining and immunohistochemical detection of antigen were found in the tissues of embryos infected by L-forms. These data suggest that L-forms exert a direct teratogenic effect on cultured mouse embryos .

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2013-05-01
2024-03-28
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References

  1. Acar J. F., Sabath L. D. 1978; Bacterial persistence in vivo: resistance or tolerance to antibiotics. Scand J Infect Dis Suppl 14:86–91[PubMed]
    [Google Scholar]
  2. Andrews W. W., Schelonka R., Waites K., Stamm A., Cliver S. P., Moser S. 2008; Genital tract methicillin-resistant Staphylococcus aureus: risk of vertical transmission in pregnant women. Obstet Gynecol 111:113–118 [View Article][PubMed]
    [Google Scholar]
  3. Bourgeois-Nicolaos N., Lucet J. C., Daubié C., Benchaba F., Rajguru M., Ruimy R., Andremont A., Armand-Lefèvre L. 2010; Maternal vaginal colonisation by Staphylococcus aureus and newborn acquisition at delivery. Paediatr Perinat Epidemiol 24:488–491 [View Article][PubMed]
    [Google Scholar]
  4. Brown R. C., Hopps H. C. 1973; Staining of bacteria in tissue sections: a reliable Gram stain method. Am J Clin Pathol 60:234–240[PubMed]
    [Google Scholar]
  5. Chatterjee A. N., Ward J. B., Perkins H. R. 1967; Synthesis of mucopeptide by L-form membranes. Nature 214:1311–1314 [View Article][PubMed]
    [Google Scholar]
  6. Chen K. T., Campbell H., Borrell L. N., Huard R. C., Saiman L., Della-Latta P. 2007; Predictors and outcomes for pregnant women with vaginal-rectal carriage of community-associated methicillin-resistant Staphylococcus aureus.. Am J Perinatol 24:235–240 [View Article][PubMed]
    [Google Scholar]
  7. Dancer S. J., Noble W. C. 1991; Nasal, axillary, and perineal carriage of Staphylococcus aureus among women: identification of strains producing epidermolytic toxin. J Clin Pathol 44:681–684 [View Article][PubMed]
    [Google Scholar]
  8. Domingue G. J. Sr, Woody H. B. 1997; Bacterial persistence and expression of disease. Clin Microbiol Rev 10:320–344[PubMed]
    [Google Scholar]
  9. Guo J., Zhang L.-S., Wang Y.-M., Yan C.-H., Huang W.-P., Wu J., Yuan H.-T., Lin B.-W., Shen J.-L., Peng S.-Q. 2011; Study of embryotoxicity of Fusarium mycotoxin butenolide using a whole rat embryo culture model. Toxicol In Vitro 25:1727–1732 [View Article][PubMed]
    [Google Scholar]
  10. Laibl V. R., Sheffield J. S., Roberts S., McIntire D. D., Trevino S., Wendel G. D. Jr 2005; Clinical presentation of community-acquired methicillin-resistant Staphylococcus aureus in pregnancy. Obstet Gynecol 106:461–465 [View Article][PubMed]
    [Google Scholar]
  11. Longo M., Zanoncelli S., Brughera M., Colombo P., Wittlin S., Vennerstrom J. L., Moehrle J., Craft J. C. 2010; Comparative embryotoxicity of different antimalarial peroxides: in vitro study using the rat whole embryo culture model (WEC). Reprod Toxicol 30:583–590 [View Article][PubMed]
    [Google Scholar]
  12. Mattman L. H. 2001; Latency and persistence. In Cell Wall Deficient Forms: Stealth Pathogens, 3rd edn. pp. 155–159 Boca Raton: CRC Press;
    [Google Scholar]
  13. Mattman L. H., Tunstall L. H., Rossmoore H. W. 1961; Induction and characteristics of staphylococcal L forms. Can J Microbiol 7:705–713 [View Article][PubMed]
    [Google Scholar]
  14. Méndez S., Agulla A., Luaces J. 2011; [Vertical transmission of methicillin resistant Staphylococcus aureus]. Enferm Infecc Microbiol Clin 29:75–76 (in Spanish) [View Article][PubMed]
    [Google Scholar]
  15. Michailova L., Stoitsova S., Markova N., Kussovski V., Jordanova M., Dimova I. 2000; Interaction of alveolar macrophages with Staphylococcus aureus and induction of microbial L-forms during infection in rats. Int J Med Microbiol 290:259–267 [View Article][PubMed]
    [Google Scholar]
  16. Nakajima M., Mitsunaga K., Nakazawa K., Usami M. 2008; In vivo/in vitro study in rat embryos on indium-caused tail malformations. Reprod Toxicol 25:426–432 [View Article][PubMed]
    [Google Scholar]
  17. New D. A. T. 1978; Whole-embryo culture and the study of mammalian embryos during organogenesis. Biol Rev Camb Philos Soc 53:81–122 [View Article][PubMed]
    [Google Scholar]
  18. Pinter D. M., Mandel J., Hulten K. G., Minkoff H., Tosi M. F. 2009; Maternal–infant perinatal transmission of methicillin-resistant and methicillin-sensitive Staphylococcus aureus. Am J Perinatol 26:145–151 [View Article][PubMed]
    [Google Scholar]
  19. Sadler T. W. 1979; Culture of early somite mouse embryos during organogenesis. J Embryol Exp Morphol 49:17–25[PubMed]
    [Google Scholar]
  20. Saiman L., O’Keefe M., Graham P. L. III, Wu F., Saïd-Salim B., Kreiswirth B., LaSala A., Schlievert P. M., Della-Latta P. 2003; Hospital transmission of community-acquired methicillin-resistant Staphylococcus aureus among postpartum women. Clin Infect Dis 37:1313–1319 [View Article][PubMed]
    [Google Scholar]
  21. Tam P. P. 1998; Postimplantation mouse development: whole embryo culture and micro-manipulation. Int J Dev Biol 42:895–902[PubMed]
    [Google Scholar]
  22. Tanaka H., Nasu F., Inomata K. 1991; Fetal alcohol effects: decreased synaptic formations in the field CA3 of fetal hippocampus. Int J Dev Neurosci 9:509–517 [View Article][PubMed]
    [Google Scholar]
  23. Tung E. W., Winn L. M. 2011; Valproic acid increases formation of reactive oxygen species and induces apoptosis in postimplantation embryos: a role for oxidative stress in valproic acid-induced neural tube defects. Mol Pharmacol 80:979–987 [View Article][PubMed]
    [Google Scholar]
  24. Van Maele-Fabry G., Delhaise F., Picard J. J. 1990; Morphogenesis and quantification of the development of post-implantation mouse embryos. Toxicol In Vitro 4:149–156 [View Article][PubMed]
    [Google Scholar]
  25. Watanakunakorn C. 1979; Are wall-defective microbial variants important in clinical infectious diseases?. J Antimicrob Chemother 5:239–241 [View Article][PubMed]
    [Google Scholar]
  26. Webster W. S., Brown-Woodman P. D., Ritchie H. E. 1997; A review of the contribution of whole embryo culture to the determination of hazard and risk in teratogenicity testing. Int J Dev Biol 41:329–335[PubMed]
    [Google Scholar]
  27. Xia P. Y., Liu Y., Wang W. Y., Guan J. C. 1999; A study on vertical transmission Staphylococcus aureus L-forms infection in mice with nucleic acid hybridization in situ. Chin J Zoonoses 15:56–58
    [Google Scholar]
  28. Xu F., Guan J. C., Xia P. 2007; Immune function on mouse offspring vertical infected by Staphylococcus aureus L-forms. Chin J Zoonoses 23:1139–1142
    [Google Scholar]
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